TY  - GEN
AU  - Srinivasan, Vidhya
AU  - Sulochana, K. N.
TI  - Effect of adiponectin on expression of vascular endothelial growth factor and pigment epithelium-derived factor: : An in vitro study
PY  - 2015///
CY  - Mumbai
PB  - Wolter Kluwer
KW  - PHARMACOLOGY
N2  - This study was carried out to identify the role of adiponectin (APN) in modulating the expression of vascular endothelial growth factor (VEGF) and pigment epithelial-derived factor (PEDF) in relation to ocular angiogenesis.
Materials and Methods: 

Human retinal pigment epithelial cell lines (ARPE-19) were cultured in Dulbeco's minimum essential medium with 10% fetal bovine serum (FBS) and exposed to varying concentrations of recombinant adiponectin (5–5 ng/ml) for 1 h. Analysis of VEGF and PEDF mRNA was done by reverse transcriptase and further quantified by quantitative polymerase chain reaction. VEGF and PEDF protein expression were studied using enzyme-linked immune sorbent assay (ELISA).
Statistical Analysis: 

Unpaired Student's t-test was used to analyze the significance. P < 0.05 was accepted as statistically significant.
Results: 

ARPE cells exposed to APN showed decreased expression of VEGF mRNA, protein whereas PEDF protein is unaltered and PEDF mRNA was increased
UR  - https://journals.lww.com/iphr/fulltext/2015/47010/effect_of_adiponectin_on_expression_of_vascular.23.aspx
ER  -