TY  - GEN
AU  - Raghavan, Rahul
AU  - Cheriyamundath, Sanith
TI  - Dimethyl sulfoxide inactivates the anticancer effect of cisplatin against human myelogenous leukemia cell lines in in vitro assays
PY  - 2015///
CY  - Mumbai
PB  - Wolter Kluwer
KW  - PHARMACOLOGY
N2  - To investigate the effect of DMSO on cisplatin induced cytotoxicity (invitro) against K562 (Human mylogenous leukemia) cell line and to study the cisplatin-DMSO adduct formation using UV-spectrophotometer.
Materials and methods: 

Effect of DMSO on the cytotoxicity of cisplatin was studied in K562 (Chronic mylogenous leukemia) cell line by MTT assay. Cisplatin-DMSO adduct formation was studied by continuously monitoring the increase in absorption peaks for 30 minutes using UV-spectrophotometer.
Results: 

0.1-0.3% DMSO markedly reduced the cytotoxic activity of cisplatin in K562 cells. Cisplatin-DMSO adduct formation was detected using UV-spectrophotometer. Continuous increase in UV absorbance between 250nm-290nm was observed when cisplatin (0.5mg/ml) and DMSO (10%) were mixed
UR  - https://journals.lww.com/iphr/fulltext/2015/47030/dimethyl_sulfoxide_inactivates_the_anticancer.15.aspx
ER  -