TY  - GEN
AU  - Kulkarni, Manasi B.
AU  - Joshi, Anagha M.
AU  - Patil, Rohini V.
TI  - Novel hptlc method for simultaneous determination of co-enzyme Q10 and a-tocopherol in bulk and pharmaceutical formulation
PY  - 2018///
CY  - M. P.
PB  - Innovare Academic Sciences Pvt Ltd
KW  - PHARMACEUTICS
N2  - Objective:HPTLC Method for Simultaneous quantification of co-enzyme Q10 and α-tocopherol in bulk and capsule dosage form was developed and validated as per International Conference on Harmonization [(ICH) Q2 (R1)] guideline. Methods:The chromatograms were developed  using  a  mobile  phase  of  Toluene: ethyl acetate: chloroform (10:1:2 v/v/v)  on  Pre-coated  silica  60F254 plates and quantified by densitometric absorbance mode at 280 nm. Results:The  Rf  values  were  0.77  and  0.87  for  co-enzyme  Q10  and α-tocopherol,  respectively.  The  linearity  of  the  method  was  found  to  be  in  the  concentration range of 0.6μg-1.8 μg/band for α-tocopherol and 2 μg-6 μg/band for co-enzyme Q10. The limits of detection and quantification were 0.3154 and 0.9559 μg/band for α-tocopherol and    3.441 and 10.42 μg/band for co-enzyme Q10. Conclusion:Developed  densitometric  method  was  found  to  be  robust,  precise,  accurate,  and  rapid  and can be used to analyse  fixed-dose  capsule  samples of co-enzyme Q10 and α-tocopherol
UR  - https://innovareacademics.in/journals/index.php/ijpps/article/view/28828/15907
ER  -