Objective:
The objective of
this
study
was
to develop and validate a novel ion
-pair
liquid chromatography
method
, in order to
separate and assay
of
amlodipine
/benazepril combination in
capsules
. This method
was
a fast
, practical
and
additional choice in quality control laboratories
.
Method
s:
The
chromatographic conditions comprised of a classical C
18
-type stationary phase (
250 × 4.6 mm, 5
μ
), with a mobile phase consisting
of:
45
%
of 10
-3
Result
s:
The
method was validated for linearity
with
correlation coefficients very close to one
, the
accu
racy
with mean recovery values between
95.0
-105.0%
, precision
with
relative standard deviations of the calculated concentrations less than 5.0%
and
specificity
in the presence of
degradation products
and excipients
.
M of cetrimide
and
55%
acetonitrile
. The f
low rate was 1 ml
/mi
n;
the detection
wavelength
was
at
242
nm, under
ambient temperature
.
Conclusio
n:
The results presented in this paper show
ed
that the developed method was
fast
and
applicable
, for the separation and determination of
amlodipine
/benazepril
combination in capsules