Development of fast, precise and selective RP-HPLC methods for identification of possible degradation products of ivermectin in isolated rat hepatocytes and in different pH media
Publication details: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(2), Apr-JuneDescription: 546-552pSubject(s): Online resources: In: Indian journal of pharmaceutical education and researchSummary: Aim: A new stability indicating RP-HPLC based methods were developed to identify the possible degradation products of Ivermectin in isolated rat hepatocytes and in different pH media using a C18 RP column. Complete validation, including linearity, accuracy, recovery, precision, robustness, stability, and peak purity, was performed. Materials and Methods: HPLC system of UltiMateDionex 3000 DAD with LiChrosorb C18 Column were used in this study. During the investigation, for the removal of interfering fraction of isolated rat hepatocytes a purification procedure, consisting of protein precipitation followed by double filtration was developed. Results: Method I was applied effectively and the results indicated two major hepatic metabolites of Ivermectin. Method II was developed to monitor the chemical stability in close to physiological conditions. The appearance of a new peak and the proportional decrease in the sample concentration indicate a liability in buffer with pH12. Conclusion: The obtained results demonstrated that the degradation products of Ivermectin in hepatic cells and in alkali media are different.| Item type | Current library | Status | Barcode | |
|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-1447 |
Aim: A new stability indicating RP-HPLC based methods were developed to identify the
possible degradation products of Ivermectin in isolated rat hepatocytes and in different
pH media using a C18 RP column. Complete validation, including linearity, accuracy,
recovery, precision, robustness, stability, and peak purity, was performed. Materials
and Methods: HPLC system of UltiMateDionex 3000 DAD with LiChrosorb C18 Column
were used in this study. During the investigation, for the removal of interfering fraction
of isolated rat hepatocytes a purification procedure, consisting of protein precipitation
followed by double filtration was developed. Results: Method I was applied effectively
and the results indicated two major hepatic metabolites of Ivermectin. Method II was
developed to monitor the chemical stability in close to physiological conditions. The
appearance of a new peak and the proportional decrease in the sample concentration
indicate a liability in buffer with pH12. Conclusion: The obtained results demonstrated
that the degradation products of Ivermectin in hepatic cells and in alkali media are
different.
There are no comments on this title.
Click on an image to view it in the image viewer