Objectives: The present study is to investigate the wound healing activity of F1-PAO and
F2-PAE ointment formulations based on
Prunus amygdalus oil and extract respectively.
Materials and Methods: The ethanolic and n- hexane extract of
Prunus amygdalus
(Batsch.) Seed (Nut) was done and incorporate to make F1-PAO and F2-PAE ointments
formulations. Above formulations were investigated for their wound repairing potential
by excision and incision model in Albino wistar Rats. Animals were divided in five
groups, each containing 6 animals. Group I (control) no treatment, Group II (Negative
group) treated with simple ointment base, Group III was standard, treated with marketed
formulation (Betadine). Group IV (F1-PAO) and Group V (F2-PAE) in excision and Incision
model treated with ointments prepare from
Prunus amygdalus (Batsch.) extract and oil.
The wound healing process was examined with the naked eye until the wounds were
fully healed. In excision model, wound contraction rate while, in incision model tensile
strength (skin breaking strength) was observed and evaluated. The data obtained was
statistically verified by using one-way ANOVA followed by Dennett’s test. Results and
Discussion: Both the formulations significantly reduced the wound area and enhanced
percentage of wound closure with compared to Positive control group, but healing process
in F2-PAE was found to better than F1-PAO. Recent studies already suggested about
the phytoconstituents present in extract formulation and oil-based formulation. Alkaloid
and tannins were supposed to be present F2-PAE and responsible for better effect than
F1-PAO. Conclusion: The investigation showed that both ointment formulation i.e.,
F1-PAO and F2-PAE treated groups have significant wound healing activitiy but F2-PAE
was found to be more effective than F1-PAO.