Introduction: Apigenin (4,5,7 Trihydroxyflavone) is a natural compound of flavonoids
class, which is found in various plant constituents. Since primitive years, Apigenin has
been used as a traditional medicine, because it possesses biological functions. Common
ayurvedic formulations comprising apigenin are in market. Hence quality control of
preparation covering apigenin is desirable. Objectives: In present study, pointed to
enhance and validate UV Spectrophotometric method to evaluation of apigenin in bulk
powder. Methods: Using methanol as solvent, UV Spectrophotometric method was
developed. Apigenin showed supreme absorbance wavelength at 267 nm. New technique
enhanced and validated in terms parameters such Specificity, Discrimination, Linearity,
Correctness, Ruggedness, Solution stability as per each ICH guidelines. Results: The
detector response for apigenin was linear in the sure concentration range of 2μg/mL-
10μg/mL with correlation coefficient of 0.9995. Newly developed method was found
to be specific, Selective linear, Precise, Rugged, reproducible for estimation of apigenin
with %RSD values less than 2%. Conclusion: In this study Development and Validation
of UV-Spectrophotometric method can be employed for the apigenin assessment in bulk
powder.