CONTEXT: Vas obstruction with reversible inhibition of sperm under guidance (RISUG) for
contraception and its reversal, may cause oxidative stress or inimical effects on male reproductive
functions.
OBJECTIVE: To evaluate the biochemical and genotoxicity at the level of reactive oxygen
species (ROS) following vas occlusion with RISUG and its reversal by Dimethyl sulphoxide (DMSO)
and 5% NaHCO3 in Wistar albino rats.
SETTINGS AND DESIGN: Animals were divided into seven groups (n = 10), namely sham‑operated
control, short‑term vas occlusion with RISUG for 90 days, reversal with DMSO and 5% NaHCO3,
long‑term vas occlusion with RISUG for 360 days, reversal with DMSO and 5% NaHCO3.
MATERIALS AND METHODS: Biochemical markers in reproductive tissues, hematology, serum
biochemistry, serum electrolytes and ROS measuring indicators, e.g., lipid peroxidation, superoxide
dismutase, catalase, glutathione peroxidase, and glutathione S‑transferase were examined.
STATISTICAL ANALYSIS USED: One‑way analysis of variance test was performed for analyses of
data obtained in this study using the SPSS 10.0 software (SPSS Inc., Chicago, IL, USA).
RESULTS: The tissue and clinical chemistry did not show appreciable alterations in RISUG injected
and reversal Groups (II‑VII) as compared to sham control. The genotoxicity and various ROS markers
fluctuated within control limits following short‑ and long‑term vas occlusion and reversal.
CONCLUSIONS: The study suggested that the reversal procedures, following RISUG contraception,
were not associated with any kind of toxicological manifestations.