Diabetes mellitus (DM) is a group of metabolic disorders characterized by hyperglycemia. Diabetes mellitus is a silent killer because sufferers are often not aware of it when it is realized, complications have occurred. Treatment for this disease must be done for life to control blood sugar in the body; however, oral antidiabetic drugs often produce unwanted side effects such as bloating, diarrhea, and stomach cramps. One of the treatments for diabetes is to find sources of treatment using natural ingredients that are relatively safe, including using plants as medicines. Based on several studies, Binahong leaves (Anredera cordifolia Ten. Steenis), brotowali (Tinospora crispa L.), and cherry (Muntingia calabura L.) are medicinal plants that can be used to reduce blood sugar levels. This study aims to test the antidiabetic activity using in vivo and in vitro testing methods of extracts of binahong leaves, cherry leaves, brotowali stems and their combinations.