Natural fiber reinforced concrete can meet worldwide demand and promote sustainable development. This study aims to find out the effect of jute fiber on the mechanical properties of fresh and hardened concrete. For this purpose, cubes, cylinders and prisms were casted with two different lengths of jute fibers, i.e. 10 mm and 20 mm and five different percentage volume additions 0, 0.25, 0.50, 0.75 and 1 %. The compressive strength of jute fiber reinforced concrete was computed at 7, 28, 56 and 90 days. The split tensile and flexural strength tests was carried out at 90 days of testing. The correlation also stabilized between compressive and flexural strength of concrete. The results were noted down for different fiber length for the early and later ages of testing. The experimental data shows that adding 0.5 % jute fiber gives similar results as compared to the control mix. However, a smaller amount 0.25 % of jute fiber made the hardened properties of the concrete better. The results of the factorial analysis showed that the length and volume of the fibers have an impact on the properties of the hardened concrete. The slump value decreases with the addition of jute fiber. However, it can be maintained by using a suitable amount of super plasticizer. The correlation between compressive and flexural strength was given by fck = 7.381fct + 5.7035 with R2 value as 0.8194 and the correlation between compressive and split tensile strength was found to be fck = 7.1614ft + 6.4129 with R2 value equal to 0.6121.