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Advancement in high-performance liquid chromatography techniques : a comprehensive review

By: Contributor(s): Publication details: Raipur Asian Pharma Press 2024Edition: Vol.14(2), Apr-JunDescription: 95-103pSubject(s): Online resources: In: Asian journal of pharmaceutical analysisSummary: Today, chromatography is the backbone of separation science and is being used in all research laboratories and pharmaceutical industries of the world. In these chromatography techniques, HPLC is one of the chromatographic techniques, which is mostly used analytical technique. Recent developments in chromatographic supports and instrumentation for liquid chromatography (LC) are enabling rapid and highly efficient separations. This article represents a brief review of HPLC along with its principle and instrumentation. It describes about new trends in HPLC such as RRLC, UPLC, UFLC and Nano LC. In this article mainly focus on detailed comparison of new developments of HPLC in terms of instrumental operating conditions, applications and advantages over HPLC of each technique. Keywords: Liquid chromatography, HPLC, RRLC, UPLC, UFLC, Nano LC.
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Today, chromatography is the backbone of separation science and is being used in all research laboratories and pharmaceutical industries of the world. In these chromatography techniques, HPLC is one of the chromatographic techniques, which is mostly used analytical technique. Recent developments in chromatographic supports and instrumentation for liquid chromatography (LC) are enabling rapid and highly efficient separations. This article represents a brief review of HPLC along with its principle and instrumentation. It describes about new trends in HPLC such as RRLC, UPLC, UFLC and Nano LC. In this article mainly focus on detailed comparison of new developments of HPLC in terms of instrumental operating conditions, applications and advantages over HPLC of each technique. Keywords: Liquid chromatography, HPLC, RRLC, UPLC, UFLC, Nano LC.

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