The combination of two drugs celecoxib + tramadol and diclofenac sodium
+ tramadol with different mechanisms of action is better for achieving effective pain control.
Materials and Methods: The simple reproducible mathematically modified UV spectroscopic
methods were established for concomitant evaluation of the binary combination of celecoxib
+ tramadol and diclofenac sodium + tramadol formulations. The first technique is predicated
on separating the pure zero-order spectra of TDL and CCB from the mixture spectra and
quantification at their lambda max. The second and third method involves the ratio absorption
difference and ratio first derivative spectroscopic method for quantification of DFS and TDL.
Additionally, we followed ICH guidelines to validate these approaches for specificity, accuracy,
and precision. Results: Both formulations were effectively analyzed using the proposed
methods, with results falling into the series of 2-50 μg/mL for TDL, and 1-30 μg/mL for DFS and
CCB. The good recovery of 98.56% -101.48% with low relative error and percentage relative
standard deviation verified the methodologies' correctness and repeatability. Finally, proposed
mathematically modified spectroscopic procedures were exploited for quality control of analytes
from formulation and manually prepared mixtures. The determination of % of retrieval of an
added known quantity of authentic medications of CCB, TDL, and DFS to the powdered pill served
as additional evidence of correctness. Conclusion: The established UV spectroscopic techniques
are simple, rapid, and perfect for simultaneous quantification of CCB, DSF, and TDL from the solid
dosage forms. The assay results also confirmed the nonexistence of tablet adjuvants intervention
in the quantification of medicines in the tablets Therefore, these approaches can be utilized for
systematic quantification of these drugs in the binary formulations without chemical separation.