Effect of adiponectin on expression of vascular endothelial growth factor and pigment epithelium-derived factor : An in vitro study
Publication details: Mumbai Wolter Kluwer 2015Edition: Vol.47(1), Jan-FebDescription: 117-120pSubject(s): Online resources: In: Indian Journal of PharmacologySummary: This study was carried out to identify the role of adiponectin (APN) in modulating the expression of vascular endothelial growth factor (VEGF) and pigment epithelial-derived factor (PEDF) in relation to ocular angiogenesis. Materials and Methods: Human retinal pigment epithelial cell lines (ARPE-19) were cultured in Dulbeco's minimum essential medium with 10% fetal bovine serum (FBS) and exposed to varying concentrations of recombinant adiponectin (5–5 ng/ml) for 1 h. Analysis of VEGF and PEDF mRNA was done by reverse transcriptase and further quantified by quantitative polymerase chain reaction. VEGF and PEDF protein expression were studied using enzyme-linked immune sorbent assay (ELISA). Statistical Analysis: Unpaired Student's t-test was used to analyze the significance. P < 0.05 was accepted as statistically significant. Results: ARPE cells exposed to APN showed decreased expression of VEGF mRNA, protein whereas PEDF protein is unaltered and PEDF mRNA was increased.| Item type | Current library | Status | Barcode | |
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School of Pharmacy Archieval Section | Not for loan | 2025-1746 |
This study was carried out to identify the role of adiponectin (APN) in modulating the expression of vascular endothelial growth factor (VEGF) and pigment epithelial-derived factor (PEDF) in relation to ocular angiogenesis.
Materials and Methods:
Human retinal pigment epithelial cell lines (ARPE-19) were cultured in Dulbeco's minimum essential medium with 10% fetal bovine serum (FBS) and exposed to varying concentrations of recombinant adiponectin (5–5 ng/ml) for 1 h. Analysis of VEGF and PEDF mRNA was done by reverse transcriptase and further quantified by quantitative polymerase chain reaction. VEGF and PEDF protein expression were studied using enzyme-linked immune sorbent assay (ELISA).
Statistical Analysis:
Unpaired Student's t-test was used to analyze the significance. P < 0.05 was accepted as statistically significant.
Results:
ARPE cells exposed to APN showed decreased expression of VEGF mRNA, protein whereas PEDF protein is unaltered and PEDF mRNA was increased.
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