Piper betle L. (Piperaceae) is used in traditional medicinal diseases. Our study was planned to isolate chemical constituents from the leaves of P. betle variety desi and to characterized their structures. Phytochemical investigation of the methanolic extract of the leaves of P. betle variety desi led to isolate n-nonyl benzene (1-phenylnonane, 1), 4′-phenyl butanoxy n-hexanoate (4′-phenyl butanoxy caproate, 2), caffeic acid (3),1′-phenoloxy 5′α-hydroxyltetradecane (4) and 5-prop-2a-enyl-4,4′-(2′′α-arachidyloxy-3′′α-hydroxypropyl)-bispyrocatechol (1′′, 2′′-dihydro-2′′α-arachidyloxy-3′′α-hydroxybischavicol, 5). The structures of these phytoconstituents, isolated from this variety of P. betle for the first time, were established by spectroscopic methods and chemical constituents. The leaf essential oil was characterized by high percentage of farnesol (41.13%), acetyl eugenol (26.40%), valencene (6.34%), α - amorphene (3.43%), γ - cadinene (3.56%), isovanillin (2.27%), eugenol (1.21%), cadina-1,4-dione (1.77%) and α - bisabolene (1.15%).