Objective:HPTLC Method for Simultaneous quantification of co-enzyme Q10 and α-tocopherol in bulk and capsule dosage form was developed and validated as per International Conference on Harmonization [(ICH) Q2 (R1)] guideline. Methods:The chromatograms were developed using a mobile phase of Toluene: ethyl acetate: chloroform (10:1:2 v/v/v) on Pre-coated silica 60F254 plates and quantified by densitometric absorbance mode at 280 nm. Results:The Rf values were 0.77 and 0.87 for co-enzyme Q10 and α-tocopherol, respectively. The linearity of the method was found to be in the concentration range of 0.6μg-1.8 μg/band for α-tocopherol and 2 μg-6 μg/band for co-enzyme Q10. The limits of detection and quantification were 0.3154 and 0.9559 μg/band for α-tocopherol and 3.441 and 10.42 μg/band for co-enzyme Q10. Conclusion:Developed densitometric method was found to be robust, precise, accurate, and rapid and can be used to analyse fixed-dose capsule samples of co-enzyme Q10 and α-tocopherol.