Objective:
Tea is a widely consumed
beverage
worldwide
. The effec
t of green tea is mainly due to its high polyphenols
-(-) epigallocatechin
-3-gallate
(EGCG) content in
the
culture
of cancer cell
and bacterial cells
. The present work
was carried out to investigate the efficacy of
green tea oil (
GTO)
against
cancer cells
and bacterial cells
.
Method
s:
In this study green tea oil was prepared from green tea for different experiment and determination of fatty acids profile fro
m green tea
oil.
In the present study, peripheral blood
lymphocyte (
PBL
) was chosen as human peripher
al blood lymphocytes and blood cancer
MCF
-7 cells were
chosen as human cancer cells. To fulfill our aims and also to evaluate the activity of this phytomedicine against normal lymp
hocytes and cancer cells
the cell samples were divided
into
26
experimental
groups
in the following ways.
Each Petri dish contains 2 X 10
5
Result
s:
GTO shows a potent anticancer agent but nontoxic to normal cells. The
GTO
decrease
s the
reduced glutathione (
GSH
) level and increase
the
oxidized glutathione
(GSSG
) level signif
icantly (P
<0.05)
in MCF
-7 cells. But in lymphocytes
the GSH level
and GSSG level
were
almost
the
same
with the
control group but
doxorubicin
(DOX
) significantly decreased the GSH and increase the GSSG level.
Green tea oil treatment causes
generation
of rea
ctive
oxygen species
(ROS
) in MCF
-7 cells revealed by DCFH
cells.
2
Conclusio
n:
This
phytomedicine
ha
s a potent
anticancer activity
without damaging the normal lymph
ocytes. So, this drug can be used for further
treatment of anticancer
and antibacterial
.