| 000 | a | ||
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| 999 |
_c11210 _d11210 |
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| 003 | OSt | ||
| 005 | 20200213114544.0 | ||
| 008 | 200213b xxu||||| |||| 00| 0 eng d | ||
| 040 |
_aAIKTC-KRRC _cAIKTC-KRRC |
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| 100 |
_912179 _aGrace, A. Caroline |
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| 245 | _aANALYTICAL METHOD DEVELOPMENT FOR SIMULTANEOUS DETERMINATION OF UBIDECARENONE AND VITAMIN E ACETATE IN CAPSULE DOSAGE FORM BY HPLC | ||
| 250 | _aVol.11(1) | ||
| 260 |
_aM P _bInnovare Academic Sciences Pvt Ltd _c2019 |
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| 300 | _a79-84p. | ||
| 520 | _aObjective: To develop and val idate a new simple, accurate, precise and sensitive high performance liquid chromatographic method ( HPLC ) method for simultaneous estimation of ubidecarenone and vitamin E acetate in capsule dosage form as per international conference on harmonization (ICH ) guidelines. Method s: The chromatographic separation of drugs were achieved using hypersil C 8 Result s: The optimized method produced sharp peaks with good resolution, minimum tailing factor and satisfactory retention time were f ound to be 5.745 min and 12.565 min for vitamin E acetate and ubidecarenone respectively. The method was linear in the range of 60 -180 μg /ml for ubidecarenone and 20 -60 μg /ml for vitamin E acetate with a correlation coefficient of 0.99 9 and 0.99 93 respectively. Mean recoveries observed for ubidecarenone and vitamin E acetate were 99.85% and 99.73% respectively. The percentage relative standard deviation (% RSD ) of peak area for system precision, method preci sion , and intermediate precision were found to be less than 0.37 %. The lower degree of % RSD obtained has proved that the method was precise and robust. column (25 0 mm x 4. 6 mm , 5μ ) in isocratic elution mode with a mobile phase of methano l: ethano l: n-hexane (80:10:10 v/v/v ) at a flow rate of 1 ml /min with ultra -violet (UV ) detection at 210 nm. Conclusio n: A new simple HPLC method was developed and validated as per ICH guidelines for the simultaneous estimation of ubidecarenone and vitamin E acetate and the method can be effectively applied for the routine analysis of active pharmaceutical ingredient (API ) and formulations. | ||
| 650 | 0 |
_94639 _aPHARMACEUTICS |
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| 700 |
_912180 _aPrabha, T. |
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| 773 | 0 |
_tInternational journal of pharmacy and pharmaceutical science _x2656-0097 _dBhopal Innovare Academic Sciences Pvt Ltd |
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| 856 |
_uhttps://innovareacademics.in/journals/index.php/ijpps/article/view/30054/16549 _yClick here |
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| 942 |
_2ddc _cAR |
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