000 a
999 _c11231
_d11231
003 OSt
005 20200213150249.0
008 200213b xxu||||| |||| 00| 0 eng d
040 _aAIKTC-KRRC
_cAIKTC-KRRC
100 _912219
_aAntony, Benny
245 _aACUTE AND SUB CHRONIC TOXICITY STUDIES OF PURIFIED WITHANIA SOMNIFERA EXTRACT IN RATS
250 _aVol.10(12)
260 _aM P
_bInnovare Academic Sciences Pvt Ltd
_c2018
300 _a41-46p.
520 _aObjective: The objective of the present study was to evaluate the acute and sub -chronic ( 90 d; repeated dose ) toxicity of Withania somnifera (ashwagandha) extract in rats. Method s: The acute toxicity was evaluated as per OECD (Organisation for Economic Co -operation and Development) guidelines 423. P urified ashwagandha extract (PAE) was fed at 2000 mg/kg body weight (bw) to overnight fasted female rats. The animals were observed daily for clinical signs of abnormality/mortality. After 1 4 d , animals were sacrificed and gross pathologic al changes were recorded. Sub -chronic toxicity of PAE was studied by feeding the extract at 100, 500 and 1000 mg/kg bw daily to rats as per OECD guidelines 408 . After 9 0 d feeding , heamatological and biochemical parameters of treated rats were compared wit h control animals. Histopathology of all the major organs was also studied. Result s: In the acute toxicity study, no mortality or clinical signs of toxicity were observed in any of the animals at maximum recommended dose level of 2000 mg/kg b w; therefore the LD50 is >2000 mg/kg bw in rats. The repeated administration of PAE for 9 0 d in rats at the maximum dose level of 1000 mg/kg bw did not induce any observable toxic effects, when compared to its corresponding control animals. The hematology and biochemistr y profile of treated rats was similar to control animals and difference was non -significant (p>0.05). The histopathology of major organs of all the control and treated animals w as normal. In this study the NOAEL (No Observed Adverse Effect Level) was calcu lated as 1000 mg/kg bw daily for rats. Conclusio n: The present study clearly indicates that PAE does not have any toxic effects in animals at the dose evaluated as evidenced by acute and sub chronic toxicity studies in rats
650 0 _94639
_aPHARMACEUTICS
700 _912222
_aBenny, Merina
773 0 _dBhopal Innovare Academic Sciences Pvt Ltd
_tInternational journal of pharmacy and pharmaceutical science
_x2656-0097
856 _uhttps://innovareacademics.in/journals/index.php/ijpps/article/view/29493/16284
_yClick here
942 _2ddc
_cAR