000			a
999			_c15565 _d15565
003			OSt
005			20211123145300.0
008			211123b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_914769 _aArafa Musa
245			_aIn silico Study, Protein Kinase Inhibition and Antiproliferative Potential of Flavonoids Isolated from Bassia eriophora (Schrad.) Growing in KSA
250			_aVol.55(2), Apr-Jun
260			_aBanaglore _bAssociation of Pharmaceutical Teachers of India (APTI) _c2021
300			_a483-490p.
520			_aIntroduction: Kinase enzymes play an important role in cellular proliferation, the main target in cancer treatment is to inhibit their functions. Protein kinase inhibitors as flavonoids can be applied for prevention or treatment of cancers through inhibition of cell proliferation. Objectives: To isolate cytotoxic metabolites from B. eriophora, evaluate their antiproliferative and protein kinase inhibitory effects, as well as the in silico study for active compounds. Materials and Methods: Preparative HPLC was used for purification of the isolates. NMR, MS and UV spectroscopy were applied for characterization of the pure compounds. Sulphorhodamine-B and radiometric assay methods were employed for determination of the antiproliferative and protein kinase inhibition activities, respectively. The antiproliferative mechanism was predicted by in silico study using Molecular Operating Environment (MOE). Results: Five flavonoids; luteolin, acacetin-7-O-β-D-glucoside, diosmin, kaempferol-3-O-rutinoside and rutin were isolated and investigated for their antiproliferative and kinase inhibitory effects. Luteolin exhibited strong antiproliferative effect against certain cell lines including MCF-7, HepG2 and HCT-116 with IC50 (33.24 ± 0.83, 26.54 ±1.02 and 31.62 ±1.32 μM, respectively), while diosmin and kaempferol- 3-O-rutinoside showed strong antiproliferative effect against MCF-7 with IC50 (26.56 ± 1.12 and 28.72 ±0.98). Luteolin showed highest inhibitory effect against Aurora B and CDK4/CyclinD1 with IC50 3.16 and 4.95 m3.16 and 4.95 inhibit in slico study for the isolated metabolites against Aurora B and CDK4/CycD1 confirmed their cytotoxic profile. Conclusion: Five flavonoids were firstly isolated from B. eriophora. The putative antiproliferative mechanism of luteolin and kaempferol-3-O-rutinoside on Aurora B and CDK4/CycD1 kinases was predictable by in slico study.
650		0	_94639 _aPHARMACEUTICS
700			_914770 _a Al-Sanea, Mohammad Mahmoud
773	0		_tIndian journal of pharmaceutical education and research _dBengluru Association of Pharmaceutical Teachers of India (APTI) _x0019-5464
856			_uhttps://www.ijper.org/sites/default/files/IndJPhaEdRes-55-2-483.pdf _yClick here
942			_2ddc _cAR