000			a
999			_c15573 _d15573
003			OSt
005			20211124094534.0
008			211124b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_914783 _a Mathiyazhagan, Jayasindu
245			_a Lyophilized Fruit Juice of Citrus sinensis Triggers Mitochondria-mediated Apoptosis via Downregulation of PI3K/AKT in MCF-7 Cell Line
250			_aVol.55(2), Apr-Jun
260			_aBanaglore _bAssociation of Pharmaceutical Teachers of India (APTI) _c2021
300			_a556-565p.
520			_aBackground: Breast cancer is prevalent cancer among women and a major cause of death worldwide due to various genetic and environmental factors. Citrus sinensis is widely taken fruit juice in most countries that are believed to reduce breast cancer risk. However, the mechanism through which it acts remains unclear. Our objective was to explore the mechanism through which Citrus sinensis acts as a cytotoxic agent. Materials and Methods: FT-IR and GC-MS analyses of lyophilzed orange juice (LOJ) were performed to find phytocompounds that were subjected to in silico docking analysis against phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway. The proteins that showed the low free energy of binding was taken for gene expression analysis. RT-PCR analysis was carried out to observe PI3K/AKT/mTOR genes inhibition in LOJ treated cells. Cytotoxicity, apoptosis, caspase 9 activity were performed against the human breast cancer cell line (MCF-7). Results: The docking analysis against PI3K/AKT/mTOR with LOJ compounds showed the best interactions. Further down-regulation of PI3K/AKT and moderate up-regulation of mTOR was confirmed using gene expression analysis. LOJ showed concentration-dependent cytotoxicity, apoptosis, decreased mitochondrial membrane stability and caspase 9 against the MCF-7 cell line. Conclusion: This study concludes that LOJ can induce apoptosis in MCF-7 cells via PI3K and AKT inhibition.
650		0	_94639 _aPHARMACEUTICS
700			_914784 _a Daniel, John Arul
773	0		_x0019-5464 _tIndian journal of pharmaceutical education and research _dBengluru Association of Pharmaceutical Teachers of India (APTI)
856			_uhttps://www.ijper.org/sites/default/files/IndJPhaEdRes-55-2-556.pdf _yClick here
942			_2ddc _cAR