000			a
999			_c15574 _d15574
003			OSt
005			20211124095444.0
008			211124b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_914785 _aVadakkenchery, Zeenath Mohammed Hussain
245			_aAntiproliferative and Apoptotic Induction of Allicin in Human Lung Cancer Cell Lines
250			_aVol.55(2), Apr-Jun
260			_aBanaglore _bAssociation of Pharmaceutical Teachers of India (APTI) _c2021
300			_a566-573p.
520			_aBackground: The study relied on exploring the anticancer effects of allicin and the experimental parameters to scientifically prove its potential as a natural anticancer agent, condiment and dietary supplement present in several Allium plant varieties. Materials and Methods: In the current investigation, the efficiency of allicin was checked for its antiproliferative and apoptosis-prompting potential in the human lung cancer cell lines A549. Results: The inhibitory concentration (IC50) of allicin was 28μM against A549 cell lines. Marked apoptotic morphological changes and DNA damage were observed and visualized by standard staining. Effects of allicin on the cell cycle were determined using flow cytometry. Allicin induced apoptosis of A549 cells 24 h after treatment and decreased the number of in S-phase and indicating cell cycle arrest at G1. These results suggest that allicin killed cancer cells through apoptosis and cell cycle arrest. Further, protein and gene expressions were analyzed by western blot and RT-PCR analysis. Our results showed that, the allicin instigated apoptotic cell death in through intrinsic pathways by the activation of Caspase family proteins.
650		0	_94639 _aPHARMACEUTICS
700			_914786 _aSanthy Kumaran, Sivanandhan
773	0		_dBengluru Association of Pharmaceutical Teachers of India (APTI) _x0019-5464 _tIndian journal of pharmaceutical education and research
856			_uhttps://www.ijper.org/sites/default/files/IndJPhaEdRes-55-2-566.pdf _yClick here
942			_2ddc _cAR