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040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_917602 _aPrabhat Kumar
245			_aBinary solvent-assisted graphene synthesis by direct exfoliation of graphite powder and its cytotoxicity on siha cell line
250			_aVol.56(3), Jul-Sep
260			_aKarnataka _bAssociation of Pharmaceutical Teachers of India (APTI) _c2022
300			_a732-739p.
520			_aA cost-effective mass production of pristine graphene nanosheet is still an important challenge to harness its maximum potential in medical sciences. Here, we have used a binary solvent mixture of low dielectric constant (1,2,4- trichlorobenzene,ε = 2.24 and benzylamine,ε = 4.6) to produce a high quantity (0.6 mg/ml) of graphene under ultrasonication without compromising its quality. Obtained sample were characterized through XRD, UV-Vis, Raman, FT-IR, TEM-SAED, and cytopotency was evaluated on SiHa cell line. I G /I 2D < 1.0 (~ 0.39) was achieved on more than 250 μm2 area, indicating the uniformity and obtained I D /I G ratio was 1.21. No toxicity was found in the case of 24 hr on the SiHa cell line. EC50 computation was 84.90 μg /ml for 48 hr and 62.03 μg /ml for 72 hr. Exfoliated graphene remained stable in binary solvent even after 1 year. The proposed experiment and obtained data can be powerful tools to explore the synergic effect of binary solvent exfoliation in combination to establish graphene as a biomaterial for neuronal network, tissue engineering, drug delivery, and regenerative medicines, to name a few.
650		0	_94639 _aPHARMACEUTICS
700			_917604 _aJain, Purnima
773	0		_x0019-5464 _tIndian journal of pharmaceutical education and research _dBengluru Association of Pharmaceutical Teachers of India (APTI)
856			_uhttps://www.ijper.org/sites/default/files/IndJPhaEdRes-56-3-732.pdf _yClick here
942			_2ddc _cAR