000			a
999			_c17335 _d17335
003			OSt
005			20220826095138.0
008			220826b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_917611 _aAbdelaziz, Mazin Aboobaida Abdalla
245			_aAnticancer activity of grewia obtusa fruit on HCT- 116, MCF-7, and HeLa tumour cells besides antitubercular activity
250			_aVol.56(3), Jul-Sep
260			_aKarnataka _bAssociation of Pharmaceutical Teachers of India (APTI) _c2022
300			_a765-771p.
520			_aBackground and Objectives: Considering the unexplored therapeutic potential of a plant, Grewia obtusa (G. obtusa), and the study evaluated the biological potential of fruit extract for antitubercular and anticancer activities. Materials and Methods: G. obtusa fruit extracts were obtained by a process of cold maceration with the solvents, which includes methanol, ethyl acetate, and n-hexane. The phytochemical constituents were identified in the obtained three extracts by various qualitative tests and further screened for the activity of anticancer on HeLa, HCT- 116 and MCF-7 tumour cells, along with anti-tubercular activity against H37Rv Mycobacterium tuberculosis H37Rv Results: The results revealed that the presence of alkaloids, terpenes, lignans, carbohydrates, steroids, glycosides and flavonoids. Anticancer screening revealed the 3-4-fold efficacy of ethyl acetate extract (32.94 μg/ml) in inhibiting HCT-116 cells comparing to n-hexane (89.69 μg/ml) and methanol (128.86 μg/ml) extracts. Same result was notice on HeLa, for ethyl acetate extract with 4-5-fold potency (45.83 μg/ml) as compared to other test extracts of methanol (164.58 μg/ml) and n-hexane (194.58 μg/ml). In case of MCF-7 cell line also the GFE showed 2 to 3-fold potency (73.04 μg/ml) as compared to other test extracts of n-hexane (140.15 μg/ml) and methanol (251.79 μg/ml). The antitubercular activity showed the MIC of>200μg/ml for all extracts. Conclusion: Ethyl acetate extract exhibited more potent activity of inhibition on HCT- 116, MCF-7, and HeLa tumour cell lines than methanol and n-hexane extracts. In specifically G. obtusa fruit ethyl acetate extract was more active on HCT-116 tumour cell lines. But the antitubercular activity was not appreciable in all three extracts.
650		0	_94639 _aPHARMACEUTICS
700			_917612 _aDusthackeer, Vijayan N Azger
773	0		_x0019-5464 _dBengluru Association of Pharmaceutical Teachers of India (APTI) _tIndian journal of pharmaceutical education and research
856			_uhttps://www.ijper.org/sites/default/files/IndJPhaEdRes-56-3-765.pdf _yClick here
942			_2ddc _cAR