000			a
999			_c17373 _d17373
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008			220901b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_917678 _aAbdul Saleem Mohammad
245			_aDetermination of nitazoxanide in biological matrices by LC-MS/MS technique: method development and validation
250			_aVol.56(2), Apr-June
260			_aKarnataka _bAssociation of Pharmaceutical Teachers of India (APTI) _c2022
300			_a539-545p.
520			_aBackground: Determination of pharmaceuticals in the biological matrices is essential for toxicologic and pharmacokinetic applications. The main objective of the current work was to develop a bioanalytical method for quantifying nitazoxanide in biological samples by LC-MS/MS. Materials and Methods: Chromatographic elution of nitazoxanide and linagliptin were achieved on C18 -hypersil (5 μ, 50x4.6mm) stationary phase with mobile phase consisting of acetonitrile and 0.1% HCOOH (75:15, v/v) processed at 0.8 ml/min flow rate. Linagliptin was utilized as an internal standard (IS). Results: The method was linear in the concentration levels of 0.53–21.2 ng/ml with more than 0.999 r2 values, consisting of 0.53 ng/ml as the lower limit of quantification (LLOQ). Analytes were subjected to pretreatment by liquid-liquid extraction (LLE) procedure with average extraction recovery findings within 99.93±5.04%. Method accuracy findings were present in between 96.44% to 104.31%, and the assessed percentage relative standard deviation findings for within and between run precision were ≤ 4.68%. Conclusion: The developed LC-MS/MS procedure for quantitating nitazoxanide in the biological matrix was suitable for routine analysis of patients’ blood samples for pharmacokinetic studies and drug monitoring.
650		0	_94639 _aPHARMACEUTICS
700			_917679 _aJayanthi, B.
773	0		_tIndian journal of pharmaceutical education and research _dBengluru Association of Pharmaceutical Teachers of India (APTI) _x0019-5464
856			_uhttps://www.ijper.org/sites/default/files/IndJPhaEdRes-56-2-539.pdf _yClick here
942			_2ddc _cAR