000			a
999			_c17374 _d17374
003			OSt
005			20220901094751.0
008			220901b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_917680 _aDineva, Alexandrina
245			_aDevelopment of fast, precise and selective RP-HPLC methods for identification of possible degradation products of ivermectin in isolated rat hepatocytes and in different pH media
250			_aVol.56(2), Apr-June
260			_aKarnataka _bAssociation of Pharmaceutical Teachers of India (APTI) _c2022
300			_a546-552p.
520			_aAim: A new stability indicating RP-HPLC based methods were developed to identify the possible degradation products of Ivermectin in isolated rat hepatocytes and in different pH media using a C18 RP column. Complete validation, including linearity, accuracy, recovery, precision, robustness, stability, and peak purity, was performed. Materials and Methods: HPLC system of UltiMateDionex 3000 DAD with LiChrosorb C18 Column were used in this study. During the investigation, for the removal of interfering fraction of isolated rat hepatocytes a purification procedure, consisting of protein precipitation followed by double filtration was developed. Results: Method I was applied effectively and the results indicated two major hepatic metabolites of Ivermectin. Method II was developed to monitor the chemical stability in close to physiological conditions. The appearance of a new peak and the proportional decrease in the sample concentration indicate a liability in buffer with pH12. Conclusion: The obtained results demonstrated that the degradation products of Ivermectin in hepatic cells and in alkali media are different.
650		0	_94639 _aPHARMACEUTICS
700			_917681 _aAngelov, Borislav
773	0		_x0019-5464 _dBengluru Association of Pharmaceutical Teachers of India (APTI) _tIndian journal of pharmaceutical education and research
856			_uhttps://www.ijper.org/sites/default/files/IndJPhaEdRes-56-2-546.pdf _yClick here
942			_2ddc _cAR