000			a
999			_c17616 _d17616
003			OSt
005			20220922162459.0
008			220922b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_918056 _aRajasekaran, R.
245			_aEvaluation of cell death potential of lepidium sativum seed extracts in MCF-7 cells and molecular docking-based correlation of identified bioactive components with human caspase-6 protein
250			_aVol.56(1), Jan-Mar
260			_aKarnataka _bAssociation of Pharmaceutical Teachers of India (APTI) _c2022
300			_a166-174p.
520			_aAim and Background: The methanolic extract of Lepidium sativum L. was known for its free radical scavenging potential and anticancer properties. The aim was to perform a comparative investigation of the cytotoxic and cell death potential of the Soxhlet (SOX) and crude methanolic extract (CRU). `Materials and Methods: MTT as well as the PI-based assays in caspase-3-deficient, MCF-7 human breast cancer cells, for its cytotoxic potential and synergistic effect. LC-QTOF-MS/MS was used for characterization. These bioactive molecules were docked with human caspase-6 (2WDP) as well as its zymogen variant (4IYR) to mimicking a more physiological form of cell demise. Results: Both extracts showed a dose-dependent toxicity and the IC50 was found to be 136.75μg/ml, and 88.49 μg/ml compared with that of standard quercetin (8.72μg/ml). At their IC50 concentrations, the corresponding PI-based flow cytometry and cell death values were 31.5±3% and 32.4±5.6, while that of quercetin was 36.9±7.4 %. A synergy in cell death was seen for a combination of L. sativum extracts (CRU-MeOH & SOX-MeOH) and quercetin. Thirteen (13) compounds were reported for the first time in this seed by LC-MS/MS. Good binding affinity was seen with both human caspase -6 (2WDP) and the mutated zymogen (4IYR) with reference to that of quercetin. Conclusion: This in vitro/ in silico correlation showed that the methanolic extracts of L. sativum exhibited a significantly higher level of cell death in MCF-7 cells. Also, their synergistic increases in cell death provides a basis possibly for a combination therapy-based strategy.
650		0	_94774 _aPHARMACOLOGY
700			_918057 _aSuresh P. K.
773	0		_x0019-5464 _tIndian journal of pharmaceutical education and research _dBengluru Association of Pharmaceutical Teachers of India (APTI)
856			_uhttps://www.ijper.org/sites/default/files/IndJPhaEdRes-56-1-166.pdf _yClick here
942			_2ddc _cAR