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_aAIKTC-KRRC _cAIKTC-KRRC |
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| 100 |
_aBa, Xiao-Hong _927655 |
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| 245 | _aEffects of Ginkgo biloba extract on the apoptosis of oxygen and glucose-deprived SH-SY5Y cells and its mechanism | ||
| 250 | _aVol.47(1), Jan-Feb | ||
| 260 |
_aMumbai _bWolter Kluwer _c2015 |
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| 300 | _a101-104p. | ||
| 520 | _a Objective: The aim was to observe the effects of the extract of Ginkgo biloba (EGb761) on the apoptosis of oxygen and glucose-deprived (OGD) human neuroblastoma cells (SH-SY5Y) cells and explore its mechanism. Materials and Methods: SH-SY5Y cells were divided into normal control group, OGD group, OGD for 4 h and EGb761-pretreated groups including very low-concentration (20 μg/ml), low-concentration group (25 μg/ml), moderate-concentration group (50 μg/ml) and high-concentration group (100 μg/ml). Twenty four hours after reoxygenation, cell viability was determined with 3-[4, 5-dimehyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide assay, apoptosis rate was detected with annexin V-fluorescein isothiocyanate/propidium iodide double staining flow cytometry and the protein level of apoptosis-inducing factor (AIF) was observed with immunofluorescence technique in each group. Results: Cell viability was significantly lower in OGD group than in EGb761-pretreated groups, especially in moderate-concentration group (50 μg/ml) (P < 0.005). Apoptosis rate was significantly lower in EGb761-pretreated groups than in OGD group (P < 0.001). Immunofluorescent staining showed that there was AIF nuclear translocation in both EGb761-pretreated groups and OGD group, but AIF nuclear translocation was less in EGb761-pretreated groups than in OGD group. Conclusion: EGb761 can reduce the apoptosis of OGD SH-SY5Y cells probably through inhibiting AIF nuclear translocation. This study provides a theoretical basis for the application of EGb761 in clinical practice. | ||
| 650 | 0 |
_aPHARMACOLOGY _94774 |
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| 700 |
_aMin, Lian-Qiu _927656 |
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| 773 | 0 |
_dAndheri - Mumbai Wolters Kluwer India Private Limited _x0253-7613 _tIndian Journal of Pharmacology |
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| 856 |
_uhttps://journals.lww.com/iphr/fulltext/2015/47010/effects_of_ginkgo_biloba_extract_on_the_apoptosis.19.aspx _yClick here |
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_2ddc _cAR |
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_c23704 _d23704 |
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