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040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_aKannan, Rajaretinam Rajesh _927688
245			_aChemical genetic effects of Sargassum wightii during embryonic development in zebrafish
250			_aVol.47(2), Mar-Apr
260			_aMumbai _bWolter Kluwer _c2015
300			_a195-198p.
520			_aPhenotype based small molecule discovery is a category of chemical genetic study. The aim of this study was to observe the phytochemical based genetic effects of Sargassum wightii during organogenesis in embryonic zebrafish. Materials and Methods: The phytomolecules from S. wightii were extracted using organic solvents and treated with the 24 h old developing zebrafish embryos. The active extract was partially purified by column chromatography, C18 Sep-Pak column and reversed-phase high-performance liquid chromatography. Results: Initially, cardiac bulging was found in 2 dpf to 3 dpf (days post fertilization), then bradycardia and tubular heart were observed in the next 8 h, which also showed the reduction in the heart beat rates. The phenotypic mutation effects of bre, has, dou yan, heg and you were observed in the 3 dpf and 4 dpf of the extract treated zebrafish embryos. Conclusions: This study demonstrated that the phytomolecules from S. wightii exhibited potential molecular switches on the developmental process, which might have significant role in understanding the development based chemical genetic studies in zebrafish.
650		0	_aPHARMACOLOGY _94774
700			_aIniyan, Appadurai Muthamil _927689
773	0		_x0253-7613 _tIndian Journal of Pharmacology _dAndheri - Mumbai Wolters Kluwer India Private Limited
856			_uhttps://journals.lww.com/iphr/fulltext/2015/47020/chemical_genetic_effects_of_sargassum_wightii.13.aspx _yClick here
942			_2ddc _cAR
999			_c23735 _d23735