000			a
999			_c8617 _d8617
003			OSt
005			20190514115932.0
008			190324b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_98207 _a Talluri, Manjula
245			_a326© 2019 Indian Journal of Pharmacology Published by Wolters Kluwer - MedknowEvaluation of Rasna panchaka(indigenous drug) as oxidative stress down‑regulator using serum‑free explant culture system
250			_aVol. 50(6), November-December
260			_aMumbai _bWolter Kluwer _c2018
300			_a326-331p.
520			_aCONTEXT: The importance of phytochemicals/natural products as potential therapeutic agents in the present context is gaining a lot of importance. India with a rich heritage of such preparations needs evaluation as potent drugs. Explant culture system is a method, which is sensitive, reliable, reproducible and is capable of mimicking the in situ conditions maintaining the tissue in sufficiently high level of integration.AIM: The current study aimed to test the antioxidant activity of test compounds, namely, traditional aqueous (4212) and aqueous‑methanolic (4308) extracts of Rasnapanchaka using liver explant cultures.MATERIALS AND METHODS: Dose‑response optima of extracts (0.2–10 μg/mL) were determined using mouse liver explant culture system up to 48 h. The antioxidant property of extracts was assessed by primary oxidative defense parameters, namely, superoxide‑dismutase (SOD), catalase, reduced glutathione (GSH), and malondialdehyde (MDA).RESULTS: The results indicated that the cellular architecture of the cultured tissue was well conserved in the first 6 h with a gradual display of specific changes in the next 24 h. There was a significant increase in MDA levels in experimental groups indicating the oxidative stress induction in explants. A dose of 2.0 μg/mL extracts have shown statistically significant (P < 0.05) protection against oxidative stress. MDA levels, a measure of lipid peroxidation, were significantly (P < 0.01) reduced by 50% in extract treated explants compared to control. This effect was accompanied by the increase in the first defense enzymes SOD (50%) and catalase (18%) with no change in reduced GSH levels.CONCLUSION: The study enforces the importance of “explant culture system,” as it not only reduces the use of nonclinical/animal model but also is rapid and sensitive. Further, results of the current study also suggest that aqueous‑methanolic extract of Rasnapanchaka is having superior antioxidant activity compared to traditional water extract
650		0	_94774 _aPHARMACOLOGY
653			_aLiver explant culture
653			_aOxidative stress
653			_aRasnapanchaka
700			_98208 _a Yathapu, Srinivasa Reddy
700			_98209 _a Bharatra, Dinesh Kumar
773	0		_tIndian Journal of Pharmacology _dAndheri - Mumbai Wolters Kluwer India Private Limited _x0253-7613
856			_uhttp://www.ijp-online.com/temp/IndianJPharmacol506326-1055094_025550.pdf _yClick here
942			_2ddc _cAR