Michael, R. A. Z.
Production of chitinase from chromobacterium violaceum using agro industrial residues under solid state fermentation - Vol.83(1), Jan-Feb - Mumbai Indian Journal of Pharmaceutical Science 2021 - 39-44p.
Chitinases have huge potential applications and biological value in industries, they are used in generating
single cell proteins, sweeteners, insecticides, antifungal drugs, anti-cancer agents, biopesticides, food
processing agents, degrading agents for sea waste etc. Chitinases are used for the conversion of chitin a
polysaccharide into monomers. Chitin is produced enormously in the biosphere annually from fungal cell
walls, exoskeletons of insects and crustaceans. Chitinases producing microorganismsBacillus licheniformis,
Chromobacterium violaceum, Rhizobium radiobacter, Streptomyces griseous were obtained from microbial
type culture collection. Solid state fermentation was done on Naturally available substrates like Fish
scale waste, Prawn shell waste, Wheat bran, Potato peel, Grape leaf to check the enhanced production
of Chitinase. Prawn shell waste was found to be the best natural substrate for high activity of Chitinase
(464.98 U/g). The overall maximum yield of Chitinase, Chtinolytic activity (531.66 U/gds) was achieved
with the optimized process parameters of incubation time (60th h), temperature(34°), inoculum age (3rd d),
inoculums volume (20 % v/w), pH (7.0) moisture content 60 % v/w), 1.5 % w/w starch as Carbon source
supplement and 1 % w/w urea as Nitrogen source supplement. Both physico-chemical and nutritional
parameters had played a significant role in the production of the Chitinase enzyme.
PHARMACEUTICS
Production of chitinase from chromobacterium violaceum using agro industrial residues under solid state fermentation - Vol.83(1), Jan-Feb - Mumbai Indian Journal of Pharmaceutical Science 2021 - 39-44p.
Chitinases have huge potential applications and biological value in industries, they are used in generating
single cell proteins, sweeteners, insecticides, antifungal drugs, anti-cancer agents, biopesticides, food
processing agents, degrading agents for sea waste etc. Chitinases are used for the conversion of chitin a
polysaccharide into monomers. Chitin is produced enormously in the biosphere annually from fungal cell
walls, exoskeletons of insects and crustaceans. Chitinases producing microorganismsBacillus licheniformis,
Chromobacterium violaceum, Rhizobium radiobacter, Streptomyces griseous were obtained from microbial
type culture collection. Solid state fermentation was done on Naturally available substrates like Fish
scale waste, Prawn shell waste, Wheat bran, Potato peel, Grape leaf to check the enhanced production
of Chitinase. Prawn shell waste was found to be the best natural substrate for high activity of Chitinase
(464.98 U/g). The overall maximum yield of Chitinase, Chtinolytic activity (531.66 U/gds) was achieved
with the optimized process parameters of incubation time (60th h), temperature(34°), inoculum age (3rd d),
inoculums volume (20 % v/w), pH (7.0) moisture content 60 % v/w), 1.5 % w/w starch as Carbon source
supplement and 1 % w/w urea as Nitrogen source supplement. Both physico-chemical and nutritional
parameters had played a significant role in the production of the Chitinase enzyme.
PHARMACEUTICS