Abdou, Randa
Bioactive metabolites of aspergillus neoniger, an endophyte of the medicinal plant ficus carica - Vol.83(1), Jan-Feb - Mumbai Indian Journal of Pharmaceutical Science 2021 - 101-109p.
Endophytes are considered as a rich source of bioactive natural products. Many plants have not been
investigated for their endophytic content yet, such as the medicinal plant Ficus carica . Recent studies
confirmed antimicrobial and anticancer activities for the plant extract. To find out if its endophytes
contribute to its reported activities, the bioactive endophyte Aspergillus neoniger was selected for
investigation of its metabolites since it exerted antimicrobial and anticancer activities in preliminary
screening assays. The fungal extract was subjected to bioactivity guided chromatographic fractionation for
isolation of its bioactive metabolites. This resulted in the identification of four aurasperones (asperpyrone
D, aurasperone D, dianhydroaurasperone C, aurasperone A) through spectroscopic analysis. Aurasperone
D and asperpyrone D were found to be cytotoxic against human cervical cancer cells (50 % cytotoxic
concentration=4.4 μg ml -1 and 3.0 μg ml -1 respectively). Aurasperone D exerted strong antiproliferative
effect against human immortal erythroleukaemia cells 562 and human umbilical vein endothelial cells
(concentration at which 50 % growth inhibition achieved was 5.3 and 4.7μg ml-1) as well as asperpyrone D
(concentration at which 50 % growth inhibition achieved was 4.9 and 5.4μg ml-1). Dianhydroaurasperone
C and aurasperone A, on the contrary, showed weak cytotoxic and antiproliferative effects. All compounds
were tested for antimicrobial activity against several test strains including the plant pathogen Fusarium
oxysporum. Results revealed that aurasperone D and asperpyrone D to be most active which suggests
potential protective role of this endophyte on its host plant. These results suggest possible partial
contribution of Aspergillus neoniger to the reported activity of the host plant.
PHARMACEUTICS
Bioactive metabolites of aspergillus neoniger, an endophyte of the medicinal plant ficus carica - Vol.83(1), Jan-Feb - Mumbai Indian Journal of Pharmaceutical Science 2021 - 101-109p.
Endophytes are considered as a rich source of bioactive natural products. Many plants have not been
investigated for their endophytic content yet, such as the medicinal plant Ficus carica . Recent studies
confirmed antimicrobial and anticancer activities for the plant extract. To find out if its endophytes
contribute to its reported activities, the bioactive endophyte Aspergillus neoniger was selected for
investigation of its metabolites since it exerted antimicrobial and anticancer activities in preliminary
screening assays. The fungal extract was subjected to bioactivity guided chromatographic fractionation for
isolation of its bioactive metabolites. This resulted in the identification of four aurasperones (asperpyrone
D, aurasperone D, dianhydroaurasperone C, aurasperone A) through spectroscopic analysis. Aurasperone
D and asperpyrone D were found to be cytotoxic against human cervical cancer cells (50 % cytotoxic
concentration=4.4 μg ml -1 and 3.0 μg ml -1 respectively). Aurasperone D exerted strong antiproliferative
effect against human immortal erythroleukaemia cells 562 and human umbilical vein endothelial cells
(concentration at which 50 % growth inhibition achieved was 5.3 and 4.7μg ml-1) as well as asperpyrone D
(concentration at which 50 % growth inhibition achieved was 4.9 and 5.4μg ml-1). Dianhydroaurasperone
C and aurasperone A, on the contrary, showed weak cytotoxic and antiproliferative effects. All compounds
were tested for antimicrobial activity against several test strains including the plant pathogen Fusarium
oxysporum. Results revealed that aurasperone D and asperpyrone D to be most active which suggests
potential protective role of this endophyte on its host plant. These results suggest possible partial
contribution of Aspergillus neoniger to the reported activity of the host plant.
PHARMACEUTICS