Sirin, Salma Sultana
In vitro antileishmanial activities of three medicinal plants: argemonemexicana, murraya koenigii and cinnamomum tamala against miltefosine resistant promastigotes of leishmania donovani parasites - Vol.13(9) - M P Innovare Academic Sciences Pvt Ltd 2021 - 27-33p.
Objective: Leishmaniasis is one of the neglected tropical diseases in terms of drug development and discovery. Non-responsiveness and resistance to the drug in Leishmaniaspecies need to develop new antileishmanial potentials; herbal medicines could be the alternative one.Methods: In the present study, semi-purified fractions were prepared from the traditionally used three medicinal plants of India: Argemone mexicana (aerial shoot), Murraya koenigii (stem), and Cinnamomum tamala (bark) by using multiple solvent systems (non-polar to polar, beginning with petroleum ether followed by n-hexane, benzene, and chloroform) and an effort was given to assess the leishmanicidal activities against Leishmania donovani miltefosine resistant HePC-R (Ld/MIL-30) promastigotes in vitroand the IC50 concentrations were estimated.Results: The study revealed that the semi-purified fractions of A. mexicana, M. koenigii, and C.tamala have effective antileishmanial activities and the 50% inhibitory concentrations (IC50) are 50 μg/ml, 98 μg/ml, and 200 μg/ml, respectively. At these (IC50) concentrations, these plant semi-purified fractions were found to interfere in lipid and protein biosynthesis, alter cell morphology, DNA content, mitochondrial membrane potential, generating ROS, and apoptosis in promastigotes. The semi-purified fractions were also found noticeably non-toxic towards host splenocytes.Conclusion: These results could suggest that A. mexicana, M. koenigii, and C.tamala could carry potential novel compounds for the development of new drugs against Leishmaniasis.
PHARMACEUTICS
In vitro antileishmanial activities of three medicinal plants: argemonemexicana, murraya koenigii and cinnamomum tamala against miltefosine resistant promastigotes of leishmania donovani parasites - Vol.13(9) - M P Innovare Academic Sciences Pvt Ltd 2021 - 27-33p.
Objective: Leishmaniasis is one of the neglected tropical diseases in terms of drug development and discovery. Non-responsiveness and resistance to the drug in Leishmaniaspecies need to develop new antileishmanial potentials; herbal medicines could be the alternative one.Methods: In the present study, semi-purified fractions were prepared from the traditionally used three medicinal plants of India: Argemone mexicana (aerial shoot), Murraya koenigii (stem), and Cinnamomum tamala (bark) by using multiple solvent systems (non-polar to polar, beginning with petroleum ether followed by n-hexane, benzene, and chloroform) and an effort was given to assess the leishmanicidal activities against Leishmania donovani miltefosine resistant HePC-R (Ld/MIL-30) promastigotes in vitroand the IC50 concentrations were estimated.Results: The study revealed that the semi-purified fractions of A. mexicana, M. koenigii, and C.tamala have effective antileishmanial activities and the 50% inhibitory concentrations (IC50) are 50 μg/ml, 98 μg/ml, and 200 μg/ml, respectively. At these (IC50) concentrations, these plant semi-purified fractions were found to interfere in lipid and protein biosynthesis, alter cell morphology, DNA content, mitochondrial membrane potential, generating ROS, and apoptosis in promastigotes. The semi-purified fractions were also found noticeably non-toxic towards host splenocytes.Conclusion: These results could suggest that A. mexicana, M. koenigii, and C.tamala could carry potential novel compounds for the development of new drugs against Leishmaniasis.
PHARMACEUTICS