Yadav, Raj Narayan
Potential of ocimum sanctum to inhibit the growth of pseudomonas aeruginosa, a disease-causing micro-organism - Vol.56(3) Jul-Sep - Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022 - 816-821p.
Aim and Objectives: Plants are a good source of secondary metabolites, which have huge
potential to kill micro-organisms. The present study aimed to evaluate the antimicrobial
activity of
Ocimum sanctum ethanol extract against drug-resistant and drug-sensitive
strains of
Pseudomonas aeruginosa (
P. aeruginosa). Materials and Methods: A total of
119 strains of
P. aeruginosa, 92 multidrug-resistant (MDR) and 27 pan sensitive strains
were included in the study, which were isolated from different human clinical samples.
Ocimum sanctum crude leaves extract was prepared with the standard “cold extraction
method”. The susceptibility agar dilution plates were made by mixing molten Mueller-
Hinton Agar (MHA) media with plant extract in different quantities to get the final extract
concentration of 0.2%, 0.1% and 0.05%. These plates were used for determining the
sensitive/ resistance nature of all strains of
P. aeruginosa against plant extract. Results:
Among 92 MDR strains in MHA plates with 0.05% of plant extract, 59 strains were
sensitive and 33 were resistant. The same MDR strains in MHA plate with 0.1% of the
extract showed somewhat similar trends, 61 sensitive and 31 resistant. Whereas with
0.2% of the extract, 67 strains were sensitive, 11 showed resistance while 14 showed
discrete colonies. Among 27 pan sensitive strains at 0.05% concentration, there was
complete growth inhibition in 23, while discrete colonies were observed in 4, though, at
0.1% and 0.2%, all strains were found as sensitive. Conclusion: Analysis of the present
study showed that
Ocimum sanctum extract possess compounds with antimicrobial
properties against commonly used isolates of
Pseudomonas aeruginosa strains.
PHARMACEUTICS
Potential of ocimum sanctum to inhibit the growth of pseudomonas aeruginosa, a disease-causing micro-organism - Vol.56(3) Jul-Sep - Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022 - 816-821p.
Aim and Objectives: Plants are a good source of secondary metabolites, which have huge
potential to kill micro-organisms. The present study aimed to evaluate the antimicrobial
activity of
Ocimum sanctum ethanol extract against drug-resistant and drug-sensitive
strains of
Pseudomonas aeruginosa (
P. aeruginosa). Materials and Methods: A total of
119 strains of
P. aeruginosa, 92 multidrug-resistant (MDR) and 27 pan sensitive strains
were included in the study, which were isolated from different human clinical samples.
Ocimum sanctum crude leaves extract was prepared with the standard “cold extraction
method”. The susceptibility agar dilution plates were made by mixing molten Mueller-
Hinton Agar (MHA) media with plant extract in different quantities to get the final extract
concentration of 0.2%, 0.1% and 0.05%. These plates were used for determining the
sensitive/ resistance nature of all strains of
P. aeruginosa against plant extract. Results:
Among 92 MDR strains in MHA plates with 0.05% of plant extract, 59 strains were
sensitive and 33 were resistant. The same MDR strains in MHA plate with 0.1% of the
extract showed somewhat similar trends, 61 sensitive and 31 resistant. Whereas with
0.2% of the extract, 67 strains were sensitive, 11 showed resistance while 14 showed
discrete colonies. Among 27 pan sensitive strains at 0.05% concentration, there was
complete growth inhibition in 23, while discrete colonies were observed in 4, though, at
0.1% and 0.2%, all strains were found as sensitive. Conclusion: Analysis of the present
study showed that
Ocimum sanctum extract possess compounds with antimicrobial
properties against commonly used isolates of
Pseudomonas aeruginosa strains.
PHARMACEUTICS