Nguyen, Tu
Antimicrobial, antioxidant and cytotoxic activities of different fractions obtained from endophyticfusarium solani F01 isolated in catharanthus roseus collected in vietnam - Vol.56(2), Apr-June - Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022 - 461-469p.
Aim: Finding out the biological sources is essential for pharmaceutical field. Materials and
Methods: Endophytic fungus was isolated from
Catharanthus roseus originated in Vietnam
and then identified by comparing, phylogeny analyzing its 18S sRNA sequence using
Blast search and GenomeNet. The supernatant and sonicated mycelia were fractionated
with methanol, ethyl acetate, hexane. All the fractions were used to test antimicrobial,
antioxidant, cytotoxicity activities. Antimicrobial activities on
Staphylococcus aureus
ATCC 25923,
Pseudomonas aeruginosa ATCC 27853,
Serratia marcescens ATCC
14756,
Vibrio parahaemolyticus ATCC 17802, and
Escherichia coli ATCC 25922 were
performed by well diffusion method. In terms of antioxidant activities, reducing power
and DPPH radical scavenging assay were done. The purification from methanol extract
was performed. The purified compounds were used to test antioxidant activity. For
cytotoxicity activity, sulforhodamine B assay was used to test the extracts and purified
compounds on the breast cancer cell line (MCF-7). Results:
Fusarium solani F01 was
identified. The treated methanol extract from cell-free supernatant showed the highest
activities and wider spectrum than ethyl acetate and hexane extracts. Mycelial extracts in
these solvents did not show activities except ethyl acetate extract. The methanol crude
extract from cell-free supernatant gave the higher reducing power (1.416 ± 0.067) than
ascorbic acid (0.577 ± 0.01) while others were very low at the concentration (100 μg/
ml). Only methanol extract obtained from supernatant showed moderate scavenging
activity when comparison of their IC50 (9.17 g/ml) with IC50 of ascorbic acid (1.28 g/
ml). Four compounds were obtained that showed antioxidant activities, but there was
one purified compound showed low effects on MCF-7 while methanol extract gave the
highest inhibition (80.34 ± 1.28). Conclusion: The study suggested fungus isolated
from
Catharanthus roseus could show and give many biological compounds that will be
a potential source for pharmaceutical field.
PHARMACEUTICS
Antimicrobial, antioxidant and cytotoxic activities of different fractions obtained from endophyticfusarium solani F01 isolated in catharanthus roseus collected in vietnam - Vol.56(2), Apr-June - Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022 - 461-469p.
Aim: Finding out the biological sources is essential for pharmaceutical field. Materials and
Methods: Endophytic fungus was isolated from
Catharanthus roseus originated in Vietnam
and then identified by comparing, phylogeny analyzing its 18S sRNA sequence using
Blast search and GenomeNet. The supernatant and sonicated mycelia were fractionated
with methanol, ethyl acetate, hexane. All the fractions were used to test antimicrobial,
antioxidant, cytotoxicity activities. Antimicrobial activities on
Staphylococcus aureus
ATCC 25923,
Pseudomonas aeruginosa ATCC 27853,
Serratia marcescens ATCC
14756,
Vibrio parahaemolyticus ATCC 17802, and
Escherichia coli ATCC 25922 were
performed by well diffusion method. In terms of antioxidant activities, reducing power
and DPPH radical scavenging assay were done. The purification from methanol extract
was performed. The purified compounds were used to test antioxidant activity. For
cytotoxicity activity, sulforhodamine B assay was used to test the extracts and purified
compounds on the breast cancer cell line (MCF-7). Results:
Fusarium solani F01 was
identified. The treated methanol extract from cell-free supernatant showed the highest
activities and wider spectrum than ethyl acetate and hexane extracts. Mycelial extracts in
these solvents did not show activities except ethyl acetate extract. The methanol crude
extract from cell-free supernatant gave the higher reducing power (1.416 ± 0.067) than
ascorbic acid (0.577 ± 0.01) while others were very low at the concentration (100 μg/
ml). Only methanol extract obtained from supernatant showed moderate scavenging
activity when comparison of their IC50 (9.17 g/ml) with IC50 of ascorbic acid (1.28 g/
ml). Four compounds were obtained that showed antioxidant activities, but there was
one purified compound showed low effects on MCF-7 while methanol extract gave the
highest inhibition (80.34 ± 1.28). Conclusion: The study suggested fungus isolated
from
Catharanthus roseus could show and give many biological compounds that will be
a potential source for pharmaceutical field.
PHARMACEUTICS