Saini, A.
Erythrocyte morphology, osmotic fragility and hematological studies after short term dietary copper deficiency in male wistar rats - Vol.84(1), Jan-Feb - Mumbai Indian Journal of Pharmaceutical Science 2022 - 27-34p.
Trace element copper is involved in myriad of physiological activities vital for normal function of organs
of animals and humans from fetal development onwards. Its deficiency might occur in infants, children as
well as adults and has been reported as a global problem affecting developed and undeveloped countries.
Copper is essential for efficient iron uptake and mobilization. The present study evaluates the effect of
dietary copper deficiency on erythrocyte of prepubertal male Wistar rats for 2, 4 and 6 w. Erythrocyte
morphology, osmotic fragility, hematological parameters-hemoglobin, total leukocyte count, cell count
(neutrophil, lymphocytes monocytes, eosinophils, platelets), packed cell volume, total red blood cell
count, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin
concentration were evaluated. Occurrence of stomatocytes, echinocytes, dracocytes, acanthocytes,
Howell-Jolly inclusion, microcyte and macrocytes were observed after 2 and 4 w of copper deficiency
when compared with their respective control and pair fed groups. The extent of morphological changes
enhanced after 6 w of dietary copper deficiency with additional appearance of fragmented cells and few
elongated cells. The percentage of altered erythrocyte morphology after 2 w deficiency was 0.051 % which
enhanced subsequently as duration increased to 0.081 % (4 w) and 0.1709 % (6 w). Consequent to these
changes significant (p<0.05) increase in erythrocyte osmotic fragility, eosinophil and lymphocyte count was
observed while hemoglobin, neutrophil and platelet count, packed cell volume, total red blood cell count,
mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration
decreased. Monocyte decrease was not significant. These changes were more pronounced after 4 and 6 w.
Dietary copper insufficiency caused impairment of membrane, deregulation of hematopoiesis and altered
the hematological profile indicating detrimental changes in properties of cellular membrane which may
probably be due to enhanced oxidative stress.
PHARMACEUTICS
Erythrocyte morphology, osmotic fragility and hematological studies after short term dietary copper deficiency in male wistar rats - Vol.84(1), Jan-Feb - Mumbai Indian Journal of Pharmaceutical Science 2022 - 27-34p.
Trace element copper is involved in myriad of physiological activities vital for normal function of organs
of animals and humans from fetal development onwards. Its deficiency might occur in infants, children as
well as adults and has been reported as a global problem affecting developed and undeveloped countries.
Copper is essential for efficient iron uptake and mobilization. The present study evaluates the effect of
dietary copper deficiency on erythrocyte of prepubertal male Wistar rats for 2, 4 and 6 w. Erythrocyte
morphology, osmotic fragility, hematological parameters-hemoglobin, total leukocyte count, cell count
(neutrophil, lymphocytes monocytes, eosinophils, platelets), packed cell volume, total red blood cell
count, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin
concentration were evaluated. Occurrence of stomatocytes, echinocytes, dracocytes, acanthocytes,
Howell-Jolly inclusion, microcyte and macrocytes were observed after 2 and 4 w of copper deficiency
when compared with their respective control and pair fed groups. The extent of morphological changes
enhanced after 6 w of dietary copper deficiency with additional appearance of fragmented cells and few
elongated cells. The percentage of altered erythrocyte morphology after 2 w deficiency was 0.051 % which
enhanced subsequently as duration increased to 0.081 % (4 w) and 0.1709 % (6 w). Consequent to these
changes significant (p<0.05) increase in erythrocyte osmotic fragility, eosinophil and lymphocyte count was
observed while hemoglobin, neutrophil and platelet count, packed cell volume, total red blood cell count,
mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration
decreased. Monocyte decrease was not significant. These changes were more pronounced after 4 and 6 w.
Dietary copper insufficiency caused impairment of membrane, deregulation of hematopoiesis and altered
the hematological profile indicating detrimental changes in properties of cellular membrane which may
probably be due to enhanced oxidative stress.
PHARMACEUTICS