Yanan Liu, Lin Yang
Walnut green husk extract suppresses ovarian cancer cell proliferation, migration and invasion via modulating microRNA-144-3p - Vol.85(4), Jul-Aug - Mumbai Indian Journal of Pharmaceutical Science 2023 - 972-978p.
Previous reports have indicated that walnut green husk extract has an inhibitory effect on human cancer
development. This project aimed to explore the influence of walnut green husk extract on ovarian cancer.
SKOV3 cells were exposed to various doses of walnut green husk extract or transfected with microRNA-
control or microRNA-144-3p. Meanwhile, anti-microRNA-144-3p or anti-microRNA-control-transfected
SKOV3 cells were treated with high-dose walnut green husk extract. Cell counting kit-8, flow cytometry
and Transwell monitored cell activity, cycle progression, migration and invasion. Cyclin D1, matrix
metallopeptidase 2, matrix metallopeptidase 9, microRNA-144-3p contents were appraised via Western blot
or real-time quantitative polymerase chine reaction. After treatment with different concentrations of walnut
green husk extract, cell activity, cycle progression, migration, invasion and cyclin D1, matrix metallopeptidase
2 and matrix metallopeptidase 9 protein expression were decreased and microRNA-144-3p level increased
in a dose-dependent manner. MicroRNA-144-3p upregulation might hinder cell activity, cycle progression,
migration and invasion. Additionally, miR-144-3p knockdown might abolish the repression of high-dose
walnut green husk extract on Janus kinase 2 and signal transducer, and activator of transcription 3 expression.
Walnut green husk extract might block ovarian cancer development via modulating the microRNA-144-3p/
Janus kinase 2/signal transducer and activator of transcription 3 pathway.
PHARMACEUTICS
Walnut green husk extract suppresses ovarian cancer cell proliferation, migration and invasion via modulating microRNA-144-3p - Vol.85(4), Jul-Aug - Mumbai Indian Journal of Pharmaceutical Science 2023 - 972-978p.
Previous reports have indicated that walnut green husk extract has an inhibitory effect on human cancer
development. This project aimed to explore the influence of walnut green husk extract on ovarian cancer.
SKOV3 cells were exposed to various doses of walnut green husk extract or transfected with microRNA-
control or microRNA-144-3p. Meanwhile, anti-microRNA-144-3p or anti-microRNA-control-transfected
SKOV3 cells were treated with high-dose walnut green husk extract. Cell counting kit-8, flow cytometry
and Transwell monitored cell activity, cycle progression, migration and invasion. Cyclin D1, matrix
metallopeptidase 2, matrix metallopeptidase 9, microRNA-144-3p contents were appraised via Western blot
or real-time quantitative polymerase chine reaction. After treatment with different concentrations of walnut
green husk extract, cell activity, cycle progression, migration, invasion and cyclin D1, matrix metallopeptidase
2 and matrix metallopeptidase 9 protein expression were decreased and microRNA-144-3p level increased
in a dose-dependent manner. MicroRNA-144-3p upregulation might hinder cell activity, cycle progression,
migration and invasion. Additionally, miR-144-3p knockdown might abolish the repression of high-dose
walnut green husk extract on Janus kinase 2 and signal transducer, and activator of transcription 3 expression.
Walnut green husk extract might block ovarian cancer development via modulating the microRNA-144-3p/
Janus kinase 2/signal transducer and activator of transcription 3 pathway.
PHARMACEUTICS