EXPRESSION PROFILING OF SELECTED MICRO RNAs COUPLED WITH MOLECULAR AND BIOCHEMICAL ANALYSES OF ASIATICOSIDES IN CENTELLA ASIATICA (L.)URB IN VITRO (Record no. 11189)
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| fixed length control field | a |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20200212111550.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200212b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 12139 |
| Author | Jisha, S. |
| 245 ## - TITLE STATEMENT | |
| Title | EXPRESSION PROFILING OF SELECTED MICRO RNAs COUPLED WITH MOLECULAR AND BIOCHEMICAL ANALYSES OF ASIATICOSIDES IN CENTELLA ASIATICA (L.)URB IN VITRO |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.11(5) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | M P |
| Name of publisher, distributor, etc. | Innovare Academic Sciences Pvt Ltd |
| Year | 2019 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 19-26p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Objective : Centella asiatica (L.) Urb from Umbelliferae is a potential source o f secondary metabolites having immense medicinal va lue. Asiaticoside is the major therapeutic compound. In the present s tudy, Identification of a possible relationship bet ween concentration/transcript level expression of asiaticoside and concentrations of growth hormon es at different growth stages was observed. The cur rent study includes molecular and biochemical evaluation of stress generated in C. asiatica at different time intervals in vitro . Methods: The enhancement in auxin, cytokinin and final asiat icoside content were determined using immunoassay k its for auxin, cytokinin and HPLC analysis respectively. Transcript level express ion at different growth phases was carried out usin g real-time RT-PCR. For isolation of stress- related miRNAs, reverse transcription of total RNA using miScript II RT Kit PCR System was carried out as per instructions. The differential expression of five selected miRNAs was done by Real -Time RT-PCR. The analysis of stress in vitro was done by quantification of Hydrogen Peroxide (H 2 O 2 ), total phenolics and total antioxidants by H 2 O 2 assay kit, total antioxidant assay kit and Folin Ciocalteau reagent respectively. The final asiaticoside content was determined by HPLC. Results: Differential expression of key genes involved in as iaticoside pathway showed significantly higher tran script expression, which is in correlation with the final asiaticoside content. Th e enhanced expression of miRNAs and the analysis of H 2 O 2, total antioxidant capacity and total phenolics are suggestive of generation of oxidative stress under controlled conditions. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 12140 |
| Co-Author | Hemanthakumar, A. S. |
| 773 0# - HOST ITEM ENTRY | |
| International Standard Serial Number | 2656-0097 |
| Place, publisher, and date of publication | Bhopal Innovare Academic Sciences Pvt Ltd |
| Title | International journal of pharmacy and pharmaceutical science |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://innovareacademics.in/journals/index.php/ijpps/article/view/32097/19859 |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| School of Pharmacy | School of Pharmacy | Archieval Section | 2020-02-12 | 2020922 | 2020-02-12 | 2020-02-12 | Articles Abstract Database |