EXPRESSION PROFILING OF SELECTED MICRO RNAs COUPLED WITH MOLECULAR AND BIOCHEMICAL ANALYSES OF ASIATICOSIDES IN CENTELLA ASIATICA (L.)URB IN VITRO
By: Jisha, S
.
Contributor(s): Hemanthakumar, A. S.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol.11(5).Description: 19-26p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective
:
Centella asiatica
(L.) Urb from Umbelliferae is a potential source o
f secondary metabolites having immense medicinal va
lue. Asiaticoside
is the major therapeutic compound. In the present s
tudy, Identification of a possible relationship bet
ween concentration/transcript level expression
of asiaticoside and concentrations of growth hormon
es at different growth stages was observed. The cur
rent study includes molecular and
biochemical evaluation of stress generated in
C. asiatica
at different time intervals
in vitro
.
Methods:
The enhancement in auxin, cytokinin and final asiat
icoside content were determined using immunoassay k
its for auxin, cytokinin and
HPLC analysis respectively. Transcript level express
ion at different growth phases was carried out usin
g real-time RT-PCR. For isolation of stress-
related miRNAs, reverse transcription of total RNA
using miScript II RT Kit PCR System was carried out
as per instructions. The differential
expression of five selected miRNAs was done by Real
-Time RT-PCR. The analysis of stress
in vitro
was done by quantification of Hydrogen Peroxide
(H
2
O
2
), total phenolics and total antioxidants by H
2
O
2
assay kit, total antioxidant assay kit and
Folin Ciocalteau reagent
respectively. The final
asiaticoside content was determined by HPLC.
Results:
Differential expression of key genes involved in as
iaticoside pathway showed significantly higher tran
script expression, which is in
correlation with the final asiaticoside content. Th
e enhanced expression of miRNAs and the analysis of
H
2
O
2,
total antioxidant capacity and total
phenolics are suggestive of generation of oxidative
stress under controlled conditions.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2020922 |
Objective
:
Centella asiatica
(L.) Urb from Umbelliferae is a potential source o
f secondary metabolites having immense medicinal va
lue. Asiaticoside
is the major therapeutic compound. In the present s
tudy, Identification of a possible relationship bet
ween concentration/transcript level expression
of asiaticoside and concentrations of growth hormon
es at different growth stages was observed. The cur
rent study includes molecular and
biochemical evaluation of stress generated in
C. asiatica
at different time intervals
in vitro
.
Methods:
The enhancement in auxin, cytokinin and final asiat
icoside content were determined using immunoassay k
its for auxin, cytokinin and
HPLC analysis respectively. Transcript level express
ion at different growth phases was carried out usin
g real-time RT-PCR. For isolation of stress-
related miRNAs, reverse transcription of total RNA
using miScript II RT Kit PCR System was carried out
as per instructions. The differential
expression of five selected miRNAs was done by Real
-Time RT-PCR. The analysis of stress
in vitro
was done by quantification of Hydrogen Peroxide
(H
2
O
2
), total phenolics and total antioxidants by H
2
O
2
assay kit, total antioxidant assay kit and
Folin Ciocalteau reagent
respectively. The final
asiaticoside content was determined by HPLC.
Results:
Differential expression of key genes involved in as
iaticoside pathway showed significantly higher tran
script expression, which is in
correlation with the final asiaticoside content. Th
e enhanced expression of miRNAs and the analysis of
H
2
O
2,
total antioxidant capacity and total
phenolics are suggestive of generation of oxidative
stress under controlled conditions.
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