Dichloromethane Crude Extract of Gymnanthemum extensum Combined with Low Piperine Fractional Piper nigrum Extract Induces Apoptosis on Human Breast Cancer Cells (Record no. 15774)
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| 000 -LEADER | |
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| fixed length control field | a |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20211228094607.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 211228b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 15119 |
| Author | FAISAL, M. |
| 245 ## - TITLE STATEMENT | |
| Title | Dichloromethane Crude Extract of Gymnanthemum extensum Combined with Low Piperine Fractional Piper nigrum Extract Induces Apoptosis on Human Breast Cancer Cells |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.83(2), March-April |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Mumbai |
| Name of publisher, distributor, etc. | Indian Journal of Pharmaceutical Science |
| Year | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 247-260p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This study aims to investigate the cytotoxicity of Andrographis paniculata , Ziziphus spina-christi and Gymnanthemum extensum crude extracts and their combination with low piperine fractional Piper nigrum extract. All plants were extracted with water and five organic solvents (methanol, ethanol, dichloromethane, ethyl acetate and hexane). Cytotoxicity was assessed by 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide assay on three cancer types (breast, colorectal and ovarian cancers) and two non-cancerous cells. The combination among extracts with low piperine fractional Piper nigrum extract was separately conducted in several tests including cytotoxicity, apoptosis and multi caspase activity. We found that, the dichloromethane crude extract of Andrographis paniculata, Ziziphus spina- christi and Gymnanthemum extensum exhibited the strongest cytotoxicity oncolorectal cancer cells SW-620 (7.49±0.04 μg ml-1), breast cancer cells Michigan Cancer Foundation-7 (13.35±0.30 μg. ml-1) and ovarian cancer cells A2780 (15.58±1.81 μg ml -1), respectively. Gas chromatography mass spectrometry study of dichloromethane crude extract of Andrographis paniculata, Ziziphus spina-christi and Gymnanthemum extensum identified major compounds including 1-heptatriacotanol (60.29 %) and palmitic acid (26.92 % for dichloromethane crude extract of Ziziphus spina-christi and 21.40 % for dichloromethane crude extract of Gymnanthemum extensum ), respectively. The combination of dichloromethane crude extract of Ziziphus spina-christi and low piperine fractional Piper nigrum extract at ratio IC 50: 0.5 IC 50 showed a moderate synergistic e ffect on Michigan Cancer Foundation-7 cells. Interestingly, the mixture of dichloromethane crude extract of Gymnanthemum extensum with low piperine fractional Piper nigrum extract at ratio IC50:IC50, 0.5 IC50:IC50 and IC50:0.5 IC50 exhibited a synergistic effect on Michigan Cancer Foundation-7 cells. Moreover, combination ofdichloromethane crude extract ofGymnanthemum extensum with low piperine fractionalPiper nigrum extract induced the apoptosis and multi caspase activity in a time dependent manner. In conclusion, dichloromethane crude extract of Andrographis paniculata, Ziziphus spina-christi and Gymnanthemum extensum displayed potent anticancer activities and the combination of dichloromethane crude extract of Gymnanthemum extensum with low piperine fractional Piper nigrum extract can be a promising regimen for an alternative cancer treatment |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 15120 |
| Co-Author | MAUNGCHANBUREE, S. |
| 773 0# - HOST ITEM ENTRY | |
| Place, publisher, and date of publication | New Delhi |
| Title | Indian journal of pharmaceutical sciences |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://www.ijpsonline.com/articles/dichloromethane-crude-extract-of-emgymnanthemum-extensumem-combined-with-low-piperine-fractional-empiper-nigrumem-extrac.pdf |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Barcode | Date last seen | Price effective from | Koha item type |
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| School of Pharmacy | School of Pharmacy | Archieval Section | 2021-12-28 | 2021-2022593 | 2021-12-28 | 2021-12-28 | Articles Abstract Database |