Dichloromethane Crude Extract of Gymnanthemum extensum Combined with Low Piperine Fractional Piper nigrum Extract Induces Apoptosis on Human Breast Cancer Cells
By: FAISAL, M
.
Contributor(s): MAUNGCHANBUREE, S.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(2), March-April.Description: 247-260p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: This study aims to investigate the cytotoxicity of Andrographis paniculata , Ziziphus spina-christi and
Gymnanthemum extensum crude extracts and their combination with low piperine fractional Piper
nigrum extract. All plants were extracted with water and five organic solvents (methanol, ethanol,
dichloromethane, ethyl acetate and hexane). Cytotoxicity was assessed by 3-(4,5-dimethylthiazol-2-yl)-
2,5-diphenyl tetrazolium bromide assay on three cancer types (breast, colorectal and ovarian cancers)
and two non-cancerous cells. The combination among extracts with low piperine fractional Piper nigrum
extract was separately conducted in several tests including cytotoxicity, apoptosis and multi caspase
activity. We found that, the dichloromethane crude extract of Andrographis paniculata, Ziziphus spina-
christi and Gymnanthemum extensum exhibited the strongest cytotoxicity oncolorectal cancer cells SW-620
(7.49±0.04 μg ml-1), breast cancer cells Michigan Cancer Foundation-7 (13.35±0.30 μg. ml-1) and ovarian
cancer cells A2780 (15.58±1.81 μg ml -1), respectively. Gas chromatography mass spectrometry study of
dichloromethane crude extract of Andrographis paniculata, Ziziphus spina-christi and Gymnanthemum
extensum identified major compounds including 1-heptatriacotanol (60.29 %) and palmitic acid
(26.92 % for dichloromethane crude extract of Ziziphus spina-christi and 21.40 % for dichloromethane
crude extract of Gymnanthemum extensum ), respectively. The combination of dichloromethane crude
extract of Ziziphus spina-christi and low piperine fractional Piper nigrum extract at ratio IC 50: 0.5 IC 50
showed a moderate synergistic e ffect on Michigan Cancer Foundation-7 cells. Interestingly, the mixture
of dichloromethane crude extract of Gymnanthemum extensum with low piperine fractional Piper nigrum
extract at ratio IC50:IC50, 0.5 IC50:IC50 and IC50:0.5 IC50 exhibited a synergistic effect on Michigan Cancer
Foundation-7 cells. Moreover, combination ofdichloromethane crude extract ofGymnanthemum extensum
with low piperine fractionalPiper nigrum extract induced the apoptosis and multi caspase activity in a time
dependent manner. In conclusion, dichloromethane crude extract of Andrographis paniculata, Ziziphus
spina-christi and Gymnanthemum extensum displayed potent anticancer activities and the combination of
dichloromethane crude extract of Gymnanthemum extensum with low piperine fractional Piper nigrum
extract can be a promising regimen for an alternative cancer treatment
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
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Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2021-2022593 |
This study aims to investigate the cytotoxicity of Andrographis paniculata , Ziziphus spina-christi and
Gymnanthemum extensum crude extracts and their combination with low piperine fractional Piper
nigrum extract. All plants were extracted with water and five organic solvents (methanol, ethanol,
dichloromethane, ethyl acetate and hexane). Cytotoxicity was assessed by 3-(4,5-dimethylthiazol-2-yl)-
2,5-diphenyl tetrazolium bromide assay on three cancer types (breast, colorectal and ovarian cancers)
and two non-cancerous cells. The combination among extracts with low piperine fractional Piper nigrum
extract was separately conducted in several tests including cytotoxicity, apoptosis and multi caspase
activity. We found that, the dichloromethane crude extract of Andrographis paniculata, Ziziphus spina-
christi and Gymnanthemum extensum exhibited the strongest cytotoxicity oncolorectal cancer cells SW-620
(7.49±0.04 μg ml-1), breast cancer cells Michigan Cancer Foundation-7 (13.35±0.30 μg. ml-1) and ovarian
cancer cells A2780 (15.58±1.81 μg ml -1), respectively. Gas chromatography mass spectrometry study of
dichloromethane crude extract of Andrographis paniculata, Ziziphus spina-christi and Gymnanthemum
extensum identified major compounds including 1-heptatriacotanol (60.29 %) and palmitic acid
(26.92 % for dichloromethane crude extract of Ziziphus spina-christi and 21.40 % for dichloromethane
crude extract of Gymnanthemum extensum ), respectively. The combination of dichloromethane crude
extract of Ziziphus spina-christi and low piperine fractional Piper nigrum extract at ratio IC 50: 0.5 IC 50
showed a moderate synergistic e ffect on Michigan Cancer Foundation-7 cells. Interestingly, the mixture
of dichloromethane crude extract of Gymnanthemum extensum with low piperine fractional Piper nigrum
extract at ratio IC50:IC50, 0.5 IC50:IC50 and IC50:0.5 IC50 exhibited a synergistic effect on Michigan Cancer
Foundation-7 cells. Moreover, combination ofdichloromethane crude extract ofGymnanthemum extensum
with low piperine fractionalPiper nigrum extract induced the apoptosis and multi caspase activity in a time
dependent manner. In conclusion, dichloromethane crude extract of Andrographis paniculata, Ziziphus
spina-christi and Gymnanthemum extensum displayed potent anticancer activities and the combination of
dichloromethane crude extract of Gymnanthemum extensum with low piperine fractional Piper nigrum
extract can be a promising regimen for an alternative cancer treatment
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