Antitumor and cytotoxic protein component from aporrectodea longa: purification, and characterization studies (Record no. 17370)
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| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220830143709.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 220830b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 17675 |
| Author | Alotaibi, Faisal |
| 245 ## - TITLE STATEMENT | |
| Title | Antitumor and cytotoxic protein component from aporrectodea longa: purification, and characterization studies |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.56(2), Apr-June |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Karnataka |
| Name of publisher, distributor, etc. | Association of Pharmaceutical Teachers of India (APTI) |
| Year | 2022 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 511-519p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Background: Cancer, a metabolic disorder with multifactorial input, is the most complex human disease to study and find a cure. Over several decades, massive research effort leads to a better understanding molecular biology of cancer and its progression. Plants and animals both were explored for novel anti-tumor agents, and enzyme-based drugs have shown significant results. These animals evolved with an enzyme with promiscuous nature capable of catalyzing multiple biochemical reactions. Enzymes are an integral part of animal physiology and are reported excellent sources of the drug. Objectives: The study aimed to isolate, purify and characterize the protein component from Aporrectodea longa for anti-cancer potential. Materials and Methods: A. longa species earthworms were collected and processed for the extraction of total protein content. The anti-tumor protein component was purified via salt precipitation, charge-based purification, and size- based purification. SDS-PAGE was used to investigate molecular weight information. The anti-tumor activity was evaluated using different cell lines, including MCF 7, Hep G2, and HT 29 with Cisplatin, 5-fluorouracil, and tamoxifen as a standard anti-cancer drug. Results: The isolated and purified protein fraction had shown proteolytic activity against casein and fibrinogen. Protein fraction represents a serine protease and average molecular weight of nearly 42kDa. The result shows protein component possesses excellent anti- tumor activity analyzed by MTT assay. Conclusion: Anti-tumor activity of isolated and purified protein fraction from A. longa may serve as a potential candidate for anti-cancer therapy. However, molecular studies are required to explore the precise mechanism of action of candidate protein. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 773 0# - HOST ITEM ENTRY | |
| Title | Indian journal of pharmaceutical education and research |
| International Standard Serial Number | 0019-5464 |
| Place, publisher, and date of publication | Bengluru Association of Pharmaceutical Teachers of India (APTI) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://www.ijper.org/sites/default/files/IndJPhaEdRes-56-2-511.pdf |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| School of Pharmacy | School of Pharmacy | Archieval Section | 2022-08-30 | 2022-1443 | 2022-08-30 | 2022-08-30 | Articles Abstract Database |