Antitumor and cytotoxic protein component from aporrectodea longa: purification, and characterization studies
By: Alotaibi, Faisal
.
Publisher: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(2), Apr-June.Description: 511-519p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Background: Cancer, a metabolic disorder with multifactorial input, is the most complex
human disease to study and find a cure. Over several decades, massive research effort
leads to a better understanding molecular biology of cancer and its progression. Plants
and animals both were explored for novel anti-tumor agents, and enzyme-based drugs
have shown significant results. These animals evolved with an enzyme with promiscuous
nature capable of catalyzing multiple biochemical reactions. Enzymes are an integral part
of animal physiology and are reported excellent sources of the drug. Objectives: The
study aimed to isolate, purify and characterize the protein component from
Aporrectodea
longa for anti-cancer potential. Materials and Methods:
A. longa species earthworms
were collected and processed for the extraction of total protein content. The anti-tumor
protein component was purified via salt precipitation, charge-based purification, and size-
based purification. SDS-PAGE was used to investigate molecular weight information. The
anti-tumor activity was evaluated using different cell lines, including MCF 7, Hep G2,
and HT 29 with Cisplatin, 5-fluorouracil, and tamoxifen as a standard anti-cancer drug.
Results: The isolated and purified protein fraction had shown proteolytic activity against
casein and fibrinogen. Protein fraction represents a serine protease and average molecular
weight of nearly 42kDa. The result shows protein component possesses excellent anti-
tumor activity analyzed by MTT assay. Conclusion: Anti-tumor activity of isolated and
purified protein fraction from A. longa may serve as a potential candidate for anti-cancer
therapy. However, molecular studies are required to explore the precise mechanism of
action of candidate protein.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-1443 |
Background: Cancer, a metabolic disorder with multifactorial input, is the most complex
human disease to study and find a cure. Over several decades, massive research effort
leads to a better understanding molecular biology of cancer and its progression. Plants
and animals both were explored for novel anti-tumor agents, and enzyme-based drugs
have shown significant results. These animals evolved with an enzyme with promiscuous
nature capable of catalyzing multiple biochemical reactions. Enzymes are an integral part
of animal physiology and are reported excellent sources of the drug. Objectives: The
study aimed to isolate, purify and characterize the protein component from
Aporrectodea
longa for anti-cancer potential. Materials and Methods:
A. longa species earthworms
were collected and processed for the extraction of total protein content. The anti-tumor
protein component was purified via salt precipitation, charge-based purification, and size-
based purification. SDS-PAGE was used to investigate molecular weight information. The
anti-tumor activity was evaluated using different cell lines, including MCF 7, Hep G2,
and HT 29 with Cisplatin, 5-fluorouracil, and tamoxifen as a standard anti-cancer drug.
Results: The isolated and purified protein fraction had shown proteolytic activity against
casein and fibrinogen. Protein fraction represents a serine protease and average molecular
weight of nearly 42kDa. The result shows protein component possesses excellent anti-
tumor activity analyzed by MTT assay. Conclusion: Anti-tumor activity of isolated and
purified protein fraction from A. longa may serve as a potential candidate for anti-cancer
therapy. However, molecular studies are required to explore the precise mechanism of
action of candidate protein.
Click on an image to view it in the image viewer
There are no comments for this item.