Effects of l-ng-monomethyl-arginine on apoptosis of articular chondrocytes
By: Wang, Guodong
.
Contributor(s): He, Bin.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(1), Jan-Feb.Description: 134-139p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: We aimed to investigate the effects of L-NG-monomethyl-arginine on the apoptosis of articular
chondrocytes. 30 rabbits were randomly divided into six groups (n=5). The pathological changes were
detected by Hematoxylin and Eosin staining. The expression levels of synovialfluid and serum nitric oxide
were measured. The apoptosis of chondrocytes was tested using the terminal deoxynucleotidyl transferase
dUTP nick end labeling assay. The expression levels of reconstituted B-cell lymphoma-2 and human B-cell
lymphoma-2 associated X protein in cartilage tissues were detected by immunohistochemical assay. The
Mankin score of the model group was signi ficantly higher than that of the control group (p<0.01). The
scores significantly decreased in L-NG-monomethyl-arginine treatment groups (p<0.05). The expression
levels of B-cell lymphoma-2 associated X protein, apoptosis rate and index of chondrocytes, and those of
synovial fluid and serum nitric oxide were markedly higher in the model group than those in the control
group (p<0.01). However, the expression levels of B-cell lymphoma-2 in model and control groups were
similar (p>0.05). With increasing L-NG-monomethyl-arginine treatment dose, the expression level of B-cell
lymphoma-2 and B-cell lymphoma-2/B-cell lymphoma-2 associated X protein ratio were signi ficantly
elevated (p<0.05). L-NG-monomethyl-arginine reduced the expressions of synovial fluid and serum nitric
oxide, and raised B-cell lymphoma-2/B-cell lymphoma-2 associated X protein ratio, thereby inhibiting
chondrocyte apoptosis.
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Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2021-2022636 |
We aimed to investigate the effects of L-NG-monomethyl-arginine on the apoptosis of articular
chondrocytes. 30 rabbits were randomly divided into six groups (n=5). The pathological changes were
detected by Hematoxylin and Eosin staining. The expression levels of synovialfluid and serum nitric oxide
were measured. The apoptosis of chondrocytes was tested using the terminal deoxynucleotidyl transferase
dUTP nick end labeling assay. The expression levels of reconstituted B-cell lymphoma-2 and human B-cell
lymphoma-2 associated X protein in cartilage tissues were detected by immunohistochemical assay. The
Mankin score of the model group was signi ficantly higher than that of the control group (p<0.01). The
scores significantly decreased in L-NG-monomethyl-arginine treatment groups (p<0.05). The expression
levels of B-cell lymphoma-2 associated X protein, apoptosis rate and index of chondrocytes, and those of
synovial fluid and serum nitric oxide were markedly higher in the model group than those in the control
group (p<0.01). However, the expression levels of B-cell lymphoma-2 in model and control groups were
similar (p>0.05). With increasing L-NG-monomethyl-arginine treatment dose, the expression level of B-cell
lymphoma-2 and B-cell lymphoma-2/B-cell lymphoma-2 associated X protein ratio were signi ficantly
elevated (p<0.05). L-NG-monomethyl-arginine reduced the expressions of synovial fluid and serum nitric
oxide, and raised B-cell lymphoma-2/B-cell lymphoma-2 associated X protein ratio, thereby inhibiting
chondrocyte apoptosis.
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