Effects of astragaloside on biological characteristics of non-small cell lung cancer cells via the phosphatidylinositol 3-kinase/protein kinase b/ mechanistic target of rapamycin pathway
By: Liu, Yuxia
.
Contributor(s): Zhao, Yue.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(4), July-Aug.Description: 865-870p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: To evaluate the effects of astragaloside on the proliferation, invasion, migration and apoptosis of non-
small cell lung cancer A549 cells and phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of
rapamycin pathway. After A549 cells were treated with 0, 10, 20, 40, 60, 80 and 100 μmol/l astragaloside
for 24 h, the survival rate was measured by methyl thiazolyl tetrazolium assay and the half maximal
inhibitory concentration was calculated. Astragaloside at half maximal inhibitory concentration was
added. Cell invasion, migration and apoptosis were detected by transwell assay, wound healing assay
and flow cytometry, respectively. The effects of astragaloside on the expressions of phosphatidylinositol
3-kinase/protein kinase B/mechanistic target of rapamycin pathway-related proteins were detected by
western blotting. After different concentrations of astragaloside were added, cell proliferation declined with
increasing concentration. Half maximal inhibitory concentration against A549 cell proliferation was 32.2
μmol/l. Compared with blank control group; cell proliferation; invasion and migration were significantly
weakened, while apoptosis was enhanced in astragaloside group. The expressions of phosphorylation of
phosphatidylinositol 3-kinase, phosphorylation of protein kinase B and phosphorylation of mechanistic
target of rapamycin proteins in astragaloside group were significantly lower than those in blank control
group. Astragaloside inhibits the proliferation, invasion and migration, and promotes the apoptosis of non-
small cell lung cancer A549 cells dose-dependently. Inhibiting phosphorylation of phosphatidylinositol
3-kinase, phosphorylation of protein kinase B and phosphorylation of mechanistic target of rapamycin
expressions in the phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin pathway
may be one of the mechanisms of astragaloside.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-0940 |
To evaluate the effects of astragaloside on the proliferation, invasion, migration and apoptosis of non-
small cell lung cancer A549 cells and phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of
rapamycin pathway. After A549 cells were treated with 0, 10, 20, 40, 60, 80 and 100 μmol/l astragaloside
for 24 h, the survival rate was measured by methyl thiazolyl tetrazolium assay and the half maximal
inhibitory concentration was calculated. Astragaloside at half maximal inhibitory concentration was
added. Cell invasion, migration and apoptosis were detected by transwell assay, wound healing assay
and flow cytometry, respectively. The effects of astragaloside on the expressions of phosphatidylinositol
3-kinase/protein kinase B/mechanistic target of rapamycin pathway-related proteins were detected by
western blotting. After different concentrations of astragaloside were added, cell proliferation declined with
increasing concentration. Half maximal inhibitory concentration against A549 cell proliferation was 32.2
μmol/l. Compared with blank control group; cell proliferation; invasion and migration were significantly
weakened, while apoptosis was enhanced in astragaloside group. The expressions of phosphorylation of
phosphatidylinositol 3-kinase, phosphorylation of protein kinase B and phosphorylation of mechanistic
target of rapamycin proteins in astragaloside group were significantly lower than those in blank control
group. Astragaloside inhibits the proliferation, invasion and migration, and promotes the apoptosis of non-
small cell lung cancer A549 cells dose-dependently. Inhibiting phosphorylation of phosphatidylinositol
3-kinase, phosphorylation of protein kinase B and phosphorylation of mechanistic target of rapamycin
expressions in the phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin pathway
may be one of the mechanisms of astragaloside.
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