Quercetin regulates inflammatory response of retinal ganglion cells by affecting the tumor necrosis factor receptor associated factor 6/transforming growth factor-β-activated kinase-1 signaling pathway
By: Deng, Zifeng
.
Contributor(s): Xiong, Shibo.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(6), Nov-Dec.Description: 1304-1308p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: To investigate the protective effect of quercetin on the lipopolysaccharide-induced inflammatory response
of retinal ganglion cell line 5 cells and to explore its possible molecular mechanism. Retinal ganglion cell
line 5 cells were cultured in vitro and assigned into blank control group, lipopolysaccharide (1.0 μg/ml)
induction group, lipopolysaccharide+low dose (0.25 μg/ml) quercetin group, lipopolysaccharide+medium
dose (0.5 μg/ml) quercetin group and lipopolysaccharide+high dose (1.0 μg/ml) quercetin group. The
relative survival rate of retinal ganglion cell line 5 cells was determined by cell counting kit-8 assay and the
levels of inflammatory factors interleukin-6 and tumor necrosis factor-alpha were detected using enzyme-
linked immunosorbent assay kits. In addition, the levels of tumor necrosis factor receptor-associated factor
6, phosphorylation-transforming growth factor-beta-activated kinase 1 and transforming growth factor-
beta-activated kinase 1 were measured by western blotting. The results of cell counting kit-8 assay revealed
that quercetin could dramatically elevate the relative survival rate of retinal ganglion cell line 5 cells. The
levels of inflammatory factors interleukin-6 and tumor necrosis factor-alpha in different quercetin groups
were significantly lower than those in lipopolysaccharide induction group and the levels of interleukin-6
and tumor necrosis factor-alpha in cell supernatant declined with the increase of quercetin concentration
in a concentration-dependent manner. In addition, the expression levels of tumor necrosis factor receptor-
associated factor 6 and phosphorylation-transforming growth factor-beta-activated kinase 1 in different
quercetin groups were remarkably lower than those in lipopolysaccharide induction group and they also
declined with the increase of quercetin concentration in a concentration-dependent manner. Quercetin
displays a protective effect against the lipopolysaccharide-induced inflammatory response of retinal
ganglion cell line 5 cells and its mechanism is related to suppression of the activation of the tumor necrosis
factor receptor-associated factor 6/transforming growth factor-beta-activated kinase 1 signaling pathway.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-1255 |
To investigate the protective effect of quercetin on the lipopolysaccharide-induced inflammatory response
of retinal ganglion cell line 5 cells and to explore its possible molecular mechanism. Retinal ganglion cell
line 5 cells were cultured in vitro and assigned into blank control group, lipopolysaccharide (1.0 μg/ml)
induction group, lipopolysaccharide+low dose (0.25 μg/ml) quercetin group, lipopolysaccharide+medium
dose (0.5 μg/ml) quercetin group and lipopolysaccharide+high dose (1.0 μg/ml) quercetin group. The
relative survival rate of retinal ganglion cell line 5 cells was determined by cell counting kit-8 assay and the
levels of inflammatory factors interleukin-6 and tumor necrosis factor-alpha were detected using enzyme-
linked immunosorbent assay kits. In addition, the levels of tumor necrosis factor receptor-associated factor
6, phosphorylation-transforming growth factor-beta-activated kinase 1 and transforming growth factor-
beta-activated kinase 1 were measured by western blotting. The results of cell counting kit-8 assay revealed
that quercetin could dramatically elevate the relative survival rate of retinal ganglion cell line 5 cells. The
levels of inflammatory factors interleukin-6 and tumor necrosis factor-alpha in different quercetin groups
were significantly lower than those in lipopolysaccharide induction group and the levels of interleukin-6
and tumor necrosis factor-alpha in cell supernatant declined with the increase of quercetin concentration
in a concentration-dependent manner. In addition, the expression levels of tumor necrosis factor receptor-
associated factor 6 and phosphorylation-transforming growth factor-beta-activated kinase 1 in different
quercetin groups were remarkably lower than those in lipopolysaccharide induction group and they also
declined with the increase of quercetin concentration in a concentration-dependent manner. Quercetin
displays a protective effect against the lipopolysaccharide-induced inflammatory response of retinal
ganglion cell line 5 cells and its mechanism is related to suppression of the activation of the tumor necrosis
factor receptor-associated factor 6/transforming growth factor-beta-activated kinase 1 signaling pathway.
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