Anticancer activity of grewia obtusa fruit on HCT- 116, MCF-7, and HeLa tumour cells besides antitubercular activity
By: Abdelaziz, Mazin Aboobaida Abdalla
.
Contributor(s): Dusthackeer, Vijayan N Azger.
Publisher: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(3), Jul-Sep.Description: 765-771p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Background and Objectives: Considering the unexplored therapeutic potential of a plant,
Grewia obtusa (G. obtusa), and the study evaluated the biological potential of fruit
extract for antitubercular and anticancer activities. Materials and Methods:
G. obtusa
fruit extracts were obtained by a process of cold maceration with the solvents, which
includes methanol, ethyl acetate, and n-hexane. The phytochemical constituents were
identified in the obtained three extracts by various qualitative tests and further screened
for the activity of anticancer on HeLa, HCT- 116 and MCF-7 tumour cells, along with
anti-tubercular activity against
H37Rv Mycobacterium tuberculosis H37Rv Results:
The results revealed that the presence of alkaloids, terpenes, lignans, carbohydrates,
steroids, glycosides and flavonoids. Anticancer screening revealed the 3-4-fold efficacy
of ethyl acetate extract (32.94 μg/ml) in inhibiting HCT-116 cells comparing to n-hexane
(89.69 μg/ml) and methanol (128.86 μg/ml) extracts. Same result was notice on HeLa,
for ethyl acetate extract with 4-5-fold potency (45.83 μg/ml) as compared to other test
extracts of methanol (164.58 μg/ml) and n-hexane (194.58 μg/ml). In case of MCF-7 cell
line also the GFE showed 2 to 3-fold potency (73.04 μg/ml) as compared to other test
extracts of n-hexane (140.15 μg/ml) and methanol (251.79 μg/ml). The antitubercular
activity showed the MIC of>200μg/ml for all extracts. Conclusion: Ethyl acetate extract
exhibited more potent activity of inhibition on HCT- 116, MCF-7, and HeLa tumour cell
lines than methanol and n-hexane extracts. In specifically
G. obtusa fruit ethyl acetate
extract was more active on HCT-116 tumour cell lines. But the antitubercular activity
was not appreciable in all three extracts.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-1386 |
Background and Objectives: Considering the unexplored therapeutic potential of a plant,
Grewia obtusa (G. obtusa), and the study evaluated the biological potential of fruit
extract for antitubercular and anticancer activities. Materials and Methods:
G. obtusa
fruit extracts were obtained by a process of cold maceration with the solvents, which
includes methanol, ethyl acetate, and n-hexane. The phytochemical constituents were
identified in the obtained three extracts by various qualitative tests and further screened
for the activity of anticancer on HeLa, HCT- 116 and MCF-7 tumour cells, along with
anti-tubercular activity against
H37Rv Mycobacterium tuberculosis H37Rv Results:
The results revealed that the presence of alkaloids, terpenes, lignans, carbohydrates,
steroids, glycosides and flavonoids. Anticancer screening revealed the 3-4-fold efficacy
of ethyl acetate extract (32.94 μg/ml) in inhibiting HCT-116 cells comparing to n-hexane
(89.69 μg/ml) and methanol (128.86 μg/ml) extracts. Same result was notice on HeLa,
for ethyl acetate extract with 4-5-fold potency (45.83 μg/ml) as compared to other test
extracts of methanol (164.58 μg/ml) and n-hexane (194.58 μg/ml). In case of MCF-7 cell
line also the GFE showed 2 to 3-fold potency (73.04 μg/ml) as compared to other test
extracts of n-hexane (140.15 μg/ml) and methanol (251.79 μg/ml). The antitubercular
activity showed the MIC of>200μg/ml for all extracts. Conclusion: Ethyl acetate extract
exhibited more potent activity of inhibition on HCT- 116, MCF-7, and HeLa tumour cell
lines than methanol and n-hexane extracts. In specifically
G. obtusa fruit ethyl acetate
extract was more active on HCT-116 tumour cell lines. But the antitubercular activity
was not appreciable in all three extracts.
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