Identification of antioxidant and antidiabetic compounds in hedychium spicatum using TLC bioautography coupled with mass spectrometry and their in silico molecular docking studies
By: Singh, Harjeet
.
Contributor(s): Singh, Nardev.
Publisher: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(3), Jul-Sep.Description: 830-837p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Background: Traditional system of medicine has been practised since a long time to
cure various diseases. More than 60-80 percent people across the world use herbal
medicine for their health care. In the present study, TLC-MS bioautography combined
with DPPH, α-amylase and α-glucosidase bioassay were used to compare antidiabetic
and antioxidant activities in hydro alcoholic extracts of
H. spicatum and also explore
in silico molecular docking studies. Materials and Methods: In this paper, hydro-alcoholic
extract of
H. spicatum was prepared, run TLC and performed TLC- MS bioautography
to identify antidiabetic and antioxidant bioactive compounds. Results: HPTLC analysis
showed there are 10 and 5 metabolites present at 254 nm and 366 nm respectively in
H. spicatum. Further, TLC-MS bioautography studies isolate total two lead compounds
such as quercetin and beta-sitosterol. Quercetin shows DPPH, α-amylase and
α-glucosidase activity whereas beta sitosterol shows α amylase activity only. Conclusion:
In conclusion, TLC-MS bioautography proved to be a good technique to evaluate the
antidiabetic and antioxidant potential of plant extracts and will be useful for discovering
new therapeutic regimens.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-1396 |
Background: Traditional system of medicine has been practised since a long time to
cure various diseases. More than 60-80 percent people across the world use herbal
medicine for their health care. In the present study, TLC-MS bioautography combined
with DPPH, α-amylase and α-glucosidase bioassay were used to compare antidiabetic
and antioxidant activities in hydro alcoholic extracts of
H. spicatum and also explore
in silico molecular docking studies. Materials and Methods: In this paper, hydro-alcoholic
extract of
H. spicatum was prepared, run TLC and performed TLC- MS bioautography
to identify antidiabetic and antioxidant bioactive compounds. Results: HPTLC analysis
showed there are 10 and 5 metabolites present at 254 nm and 366 nm respectively in
H. spicatum. Further, TLC-MS bioautography studies isolate total two lead compounds
such as quercetin and beta-sitosterol. Quercetin shows DPPH, α-amylase and
α-glucosidase activity whereas beta sitosterol shows α amylase activity only. Conclusion:
In conclusion, TLC-MS bioautography proved to be a good technique to evaluate the
antidiabetic and antioxidant potential of plant extracts and will be useful for discovering
new therapeutic regimens.
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