Experimental design approach for validation and optimisation of spectrophotometric determination of cefixime in pharmaceutical dosage form
By: Basavaraj, H
.
Contributor(s): Mruthyunjayaswamy, B. H. M
.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(1), Jan-Feb.Description: 115-120p.Subject(s): PHARMACEUTICS![](/opac-tmpl/bootstrap/images/filefind.png)
Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
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School of Pharmacy Archieval Section | Not for loan | 2022-2244 |
Two simple, sensitive and precise spectrophotometric methods for the assay of cefixime in either pure form
or in its pharmaceutical dosage form are described. The method I is based on the reaction of salicylaldehyde
with cefixime resulting in a yellow coloured product, absorbs at λmax 425 nm. The second method describes
the reaction between the diazotized drug and N-(1-naphthyl)ethylenediamine dihydrochloride to yield a
purple coloured product with λmax at 567 nm. The reaction conditions were optimized to get maximum
colour intensity. The absorbance was found to extend linearly with increasing the concentration of cefixime
the systems obeyed the Beer’s law within the range of 2-10 μg/ml and 5-25 μg/ml for salicylaldehyde
and N-(1-naphthyl)ethylenediamine dihydrochloride methods. Common excipients used as additives in
pharmaceutical dosage don’t interfere within the proposed analytical methods. The products are stable
for over 6 h and 10 h respectively. The proposed methods are simple, sensitive, accurate and suitable for
quality control uses.
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