Alpha-amylase inhibitory and antioxidant activities in aqueous acetone extract and its fractions from ampelocissus martini root
By: Siripipatthana, Patthraporn
.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol84(4), Jul-Aug.Description: 929-937p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: This study aimed to evaluate and correlate phytochemical content, antioxidant activity and alpha-amylase
inhibitory activity in aqueous acetone extract and fractions derived from it (ethyl acetate fraction, water-
soluble fraction, aqueous methanol fraction and aqueous acetone fraction) which are obtained from
Ampelocissus martini Planch. root. Ethyl acetate fraction and aqueous acetone fraction had the highest
total phenolic content and total proanthocyanidin content, respectively. 2,2-diphenyl-1-picrylhydrazyl,
2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid), cupric reducing antioxidant capacity and ferric
reducing antioxidant power assays showed that aqueous acetone fraction had higher antioxidant activity
compared to other samples and standards. Acarbose and all samples except aqueous methanol fraction
inhibited alpha-amylase, a key enzyme linked to type 2 diabetes, in a dose-dependent manner and aqueous
acetone fraction showed the strongest inhibition (half-maximal inhibitory concentration=8.77±0.28 μg/
ml). Aqueous acetone fraction was a mixed noncompetitive inhibitor of the enzyme. Correlation analysis
showed strong positive correlations among proanthocyanidin content, antioxidant activity and alpha-
amylase inhibitory activity. These results suggest that aqueous acetone fraction, proanthocyanidins-rich
fraction from Ampelocissus martini root, may be used for effective diabetes management because of its high
potential antioxidant activity and alpha-amylase inhibitory activity.
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School of Pharmacy Archieval Section | Not for loan | 2023-0849 |
This study aimed to evaluate and correlate phytochemical content, antioxidant activity and alpha-amylase
inhibitory activity in aqueous acetone extract and fractions derived from it (ethyl acetate fraction, water-
soluble fraction, aqueous methanol fraction and aqueous acetone fraction) which are obtained from
Ampelocissus martini Planch. root. Ethyl acetate fraction and aqueous acetone fraction had the highest
total phenolic content and total proanthocyanidin content, respectively. 2,2-diphenyl-1-picrylhydrazyl,
2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid), cupric reducing antioxidant capacity and ferric
reducing antioxidant power assays showed that aqueous acetone fraction had higher antioxidant activity
compared to other samples and standards. Acarbose and all samples except aqueous methanol fraction
inhibited alpha-amylase, a key enzyme linked to type 2 diabetes, in a dose-dependent manner and aqueous
acetone fraction showed the strongest inhibition (half-maximal inhibitory concentration=8.77±0.28 μg/
ml). Aqueous acetone fraction was a mixed noncompetitive inhibitor of the enzyme. Correlation analysis
showed strong positive correlations among proanthocyanidin content, antioxidant activity and alpha-
amylase inhibitory activity. These results suggest that aqueous acetone fraction, proanthocyanidins-rich
fraction from Ampelocissus martini root, may be used for effective diabetes management because of its high
potential antioxidant activity and alpha-amylase inhibitory activity.
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