Bioprospecting of marine halophyte salicornia europaea L. and evaluation of its biological potential with special reference to anticancer activity
By: Samuel, P
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Contributor(s): Vijay, Kumar | Selvarathinam, T.
Publisher: Mumbai Indian Drug Manufacture's Association - IDMA 2018Edition: Vol. 55 (05) May.Description: 47-56.Subject(s): PHARMACEUTICS | GC-MS | Soxhtel extraction | MCF7 | MTT assay
In:
Indian drugsSummary: The study was caried out with the intention to bring out the biological prospective nature of the marine halophyte Salicornia europaea L. The bioactive phytochemicals of the halophyte were extracted. The chemicals in the crude extract were evaluated for cytotoxic activity against MCF7 cell line by MTT assay. The marine halophyte a was collected, washed and chopped into 5cm long parts and shade dried for 20 – 25 days in a dark room. The dehydrated and bleached plant material was subjected to Soxhlet extraction. Two solvents, viz methanol and ethyl acetate, were used to prepare decoction. The extracts were dried using rotary vacuum evaporator. chemical screening by GC-MS unveiled the presence of 32 compounds in ethyl acetate and 29 in methanol. The 3D structures of the phytochemicals were retrieved from Pub Chem. Cytotoxic study showed promising results. The IC50 values of the individual extracts were evaluated and ethyl acetate extract exhibit minimum IC50 value and it was estimated to be 97.9μg/mL wereas methanol extract exhibit a minimum IC50 value of 117.1μg/mL. Hence, the study concludes that S. europaea L. provides a promising source of lead compounds that could be exploited in near future to treat cancer.
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The study was caried out with the intention to bring out the biological prospective nature of the marine halophyte Salicornia europaea L. The bioactive phytochemicals of the halophyte were extracted. The chemicals in the crude extract were evaluated for cytotoxic activity against MCF7 cell line by MTT assay. The marine halophyte a was collected, washed and chopped into 5cm long parts and shade dried for 20 – 25 days in a dark room. The dehydrated and bleached plant material was subjected to Soxhlet extraction. Two solvents, viz methanol and ethyl acetate, were used to prepare decoction. The extracts were dried using rotary vacuum evaporator. chemical screening by GC-MS unveiled the presence of 32 compounds in ethyl acetate and 29 in methanol. The 3D structures of the phytochemicals were retrieved from Pub Chem. Cytotoxic study showed promising results. The IC50 values of the individual extracts were evaluated and ethyl acetate extract exhibit minimum IC50 value and it was estimated to be 97.9μg/mL wereas methanol extract exhibit a minimum IC50 value of 117.1μg/mL. Hence, the study concludes that S. europaea L. provides a promising source of lead compounds that could be exploited in near future to treat cancer.
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